Oncomine™ TCR Beta-LR Assay GX
Oncomine™ TCR Beta-LR Assay GX
Ion Torrent™

Oncomine™ TCR Beta-LR Assay GX

The Oncomine TCR Beta-LR Assay GX is designed to measure T cell diversity and clonal expansion by sequencing T-cell receptorRead more
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Catalog NumberQuantity
A4629732 samples
Catalog number A46297
Price (USD)
9,010.00
Each
Add to cart
Quantity:
32 samples
Price (USD)
9,010.00
Each
Add to cart
The Oncomine TCR Beta-LR Assay GX is designed to measure T cell diversity and clonal expansion by sequencing T-cell receptor beta chain rearrangements. The assay kit provides single-pool multiplex panel library reagents to perform up to 32 sequencing reactions. The assay is optimized for long-read sequencing on the Ion Torrent Genexus Integrated Sequencer using the Ion Torrent Genexus GX5 Chip. The Genexus Integrated Sequencer performs library preparation, sequencing, analysis, and reporting in an automated workflow that delivers results in a single day. The multi-lane sequencing chip is designed to enable cost-effective processing of libraries.

The assay is designed to efficiently capture all three complementarity-determining regions of the TCR beta chain (CDR1, CDR2, CDR3) with high accuracy. Key applications include predictive or prognostic biomarker discovery, T-cell characterization, and identification of variable gene polymorphisms. The identification of rare and abundant clones can be achieved with a flexible RNA input amount of 10 ng (minimum) up to 1 μg. The use of RNA template allows sequencing of productive and relevant variable (V), diversity (D), and joining (J) rearrangements—which enables the identification of rare clones.

The Oncomine TCR Beta-LR Assay GX supports the following features:
• Long-read RNA sequencing covering the TCR beta chain
• Detection of clones over a wide dynamic range
• Efficient workflow with 24-hr sample-to-result time
• Flexible input requirements of 10 ng to 1 μg RNA
• Compatible with fresh-frozen tissue, whole blood, and sorted cells
• Streamlined and user-friendly informatics solution with multi-sample analysis functionality

Why are immunologists and immuno-oncologists interested in T cell repertoire sequencing research?
• To characterize diversity and monitor features of T lymphocytes in blood and infiltrated tumors
• To identify variable gene polymorphisms implicated in autoimmune disease or immune-mediated adverse events
• To optimize the manufacture and function of therapeutic or engineered T cells

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Sequencing
For Use With (Equipment)Ion Torrent Genexus Sequencer
LibrariesTargeted Sequencing Library
Product LineOncomine™
Product TypeTCR Beta-LR Assay GX
Quantity32 samples
Sample TypeRNA
Shipping ConditionApproved for shipment on Wet or Dry Ice
Workflow StepLibrary Generation
Sequencing TypeTranscriptome Sequencing
Unit SizeEach
Contents & Storage
• A40282 Oncomine TCR Beta-LR Assay GX, store at -10°C to -30°C
• A40254 Ion Torrent Genexus Strip 1, store at 2–8°C
• A40253 Ion Torrent Genexus Strip 2-AS, store at -10°C to -30°C

Frequently asked questions (FAQs)

What is included in Oncomine TCR Beta-LR Assay GX?

Oncomine TCR Beta-LR Assay GX includes the following:
- TCRB‑LR RNA Pool 1 panel (8 x 2 tubes, 70 ul/tube), for 32 sequencing reactions (4 reactions/tube pair)
- Genexus Strip 1 (8 strips), for 32 sequencing reactions (4 reactions/strip)
- Genexus Strip 2-AS (8 strips), for 32 sequencing reactions (4 reactions/strip)

Note:
- The TCRB-LR RNA Pool 1 is provided in 8 pairs of tubes, where each tube pair contains one tube with the panel in position 1 and one empty uncapped tube in position 2.
- Genexus Library Strips 1 and 2-AS combo kit (Cat. No. A40252) can be purchased separately.

How many sequencing reactions can I perform with Oncomine TCR Beta-LR Assay GX?

The assay kit provides the targeted single pool multiplex panel and library reagents sufficient to perform up to 32 sequencing reactions.

How much of each sample do I need for Oncomine TCR Beta-LR Assay GX (Cat. No. A46297)?

Flexible RNA input amount of 10 ng (minimum) and up to 1 µg supports the identification of rare clones as well as abundant clones.

Which sample types are compatible with Oncomine TCR Beta-LR Assay GX (Cat. No. A46297)?

Oncomine TCR Beta-LR Assay GX is compatible with fresh-frozen tissue, whole blood, and sorted cells. The assay is optimized for long-read sequencing on the Genexus Integrated Sequencer, using the Genexus GX5 Chip.

What is the goal of Oncomine TCR Beta-LR Assay GX (Cat. No. A46297) ?

Oncomine TCR Beta-LR Assay GX is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements. It is designed to capture all three complementarity-determining regions of the TCR beta chain (CDR1, CDR2, and CDR3) with high accuracy. The assay is optimized for long-read sequencing on the Genexus Integrated Sequencer, using the Genexus GX5 Chip.