Thermo Scientific™

RapidFinder™ Halal ID Kit for RT-PCR

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Industrial & Applied Science

Simply and reliably detect pork DNA in food and feed samples by real-time PCR with the RapidFinder™ Halal ID Kit. Unlike ELISA-based methods which can be less suitable for processed food, when used with the GMO Extraction Kit (Order code: 4466336) for sample preparation, the RapidFinder Halal ID Kit detects both raw and processed DNA and provides a sensitivity down to 0.0005% pork DNA. The included positive control, which comprises 0.1% of pork DNA, can be used as a reference to set the threshold of detection of the assay.
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Support Halal label claims by testing for the presence of pork DNA in food and feed samples to verify if any adulteration or cross-contamination has occurred in purchased ingredients or during manufacture.

• Simple: real-time PCR based assay, no electrophoresis needed
• Flexible: pork DNA detected in any food or feed sample, both raw and processed
• Specific: detection of highly specific mitochondrial region from gene 16s common to all pork and wild boar with a limit of detection of 0.0005%
• Reliable: Internal Positive Control (IPC) provides process-control check for PCR inhibitors
• Fast: from sample to result in the same day

How the RapidFinder™ Halal ID Kit Works
Animal DNA detection and identification is accomplished by real-time PCR using two TaqMan™-MGB probes. The target sequence, detected in the FAM dye channel, specifically detects a mitochondrial DNA sequence specific for Sus scrofa (pork). Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.0005% pork DNA, and by comparing sample results to the control results, a threshold of detection is provided.

Sample preparation
DNA sample preparation for the RapidFinder™ Halal ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample, rather than the smaller sample sizes accommodated by most other DNA isolation procedures, minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Part of a suite of solutions for meat species detection
The RapidFinder™ Halal ID Kit is just one of our many kits available for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of beef, horse, sheep, ruminant, chicken, turkey, and poultry.

The RapidFinder™ Meat Species Identification kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

For Testing of Food and Environmental Samples Only.