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resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.
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| Catalog Number | Includes |
|---|---|
| A51970 | Assay Kit and Sample Preparation |
| A51969 | Assay Kit only |
Catalog number A51970
Price (USD)/ Each
5,400.00
-
Includes:
Assay Kit and Sample Preparation
Price (USD)/ Each
5,400.00
resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
Catalog numberA51970
Price (USD)/ Each
5,400.00
-
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics. Reliable and rapid, the resDNASEQ kit enables sensitive and specific quantitation of different fragment lengths of the E1A gene within the residual host-cell DNA. This performance helps ensure a high degree of confidence in the obtained quantitation data.
Features of the resDNASEQ Quantitative E1A DNA Fragment Length Kit include:
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, three TaqMan primer/probe mixes, and a single E1A plasmid DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
Integrated workflow solution
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.
Easy to use
With the resDNASEQ Quantitative E1A DNA Fragment Length Kit, you perform qPCR analysis to quantitate the copy numbers of three different fragment size ranges in your test samples and compare that against standard curves generated with known amounts of specific fragments of the E1A DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to E1A DNA standards, as well as step-by-step instructions.
Consistent performance
For accurate quantitation of fragments of three different lengths of E1A DNA, assay results must be unaffected by the size of non-specific DNA present in the test sample. With the resDNASEQ system you get consistent assay performance across the three E1A fragment sizes.
• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Includes master mix, three TaqMan primer/probe mixes, and a single E1A plasmid DNA standard
This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts.
Integrated workflow solution
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing. The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow. The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System. Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.
Easy to use
With the resDNASEQ Quantitative E1A DNA Fragment Length Kit, you perform qPCR analysis to quantitate the copy numbers of three different fragment size ranges in your test samples and compare that against standard curves generated with known amounts of specific fragments of the E1A DNA standard. The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to E1A DNA standards, as well as step-by-step instructions.
Consistent performance
For accurate quantitation of fragments of three different lengths of E1A DNA, assay results must be unaffected by the size of non-specific DNA present in the test sample. With the resDNASEQ system you get consistent assay performance across the three E1A fragment sizes.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodPrimer-probe
FormatKit
PCR MethodqPCR
Host CellHEK
Label or DyeFAM
Product LineresDNASEQ™
Quantity100 reactions
No. of Reactions100 Reactions
IncludesAssay Kit and Sample Preparation
Unit SizeEach
Contents & Storage
resDNASEQ E1A DNA Control
• E1A DNA Control, 6.30 x 108 copies/μL, 0.12 mL, store at –25 to –15°C
• DNA Dilution Buffer (DDB), 7 mL, store at –25 to –15°C before first use and 2−8°C after first use
resDNASEQ E1A DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 5 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• E1A DNA Short Fragment Assay Mix, 1 x 400 μL, store at –15 to –25°C, protect from light
• E1A DNA Medium Fragment Assay Mix, 1 x 660 μL, store at –15 to –25°C, protect from light
• E1A DNA Long Fragment Assay, 1 x 400 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 3 x 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
PrepSEQ Nucleic Acid Extraction Kit
• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
• Elution Buffer (1 bottle, 25 mL)
• Proteinase K Buffer (1 bottle, 50 mL)
• Magnetic Particles (2 tubes, 1.5 mL/tube)
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL). Store at or below –20°C
• PK Buffer II (1 bottle, 11 mL)
Store all at room temperature unless otherwise indicated.
PrepSEQ Residual DNA Sample Preparation Kit
• Proteinase K (20 mg/mL), 1 tube, 1.25 mL
• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL
• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube
Store all at or below –20°C.
• E1A DNA Control, 6.30 x 108 copies/μL, 0.12 mL, store at –25 to –15°C
• DNA Dilution Buffer (DDB), 7 mL, store at –25 to –15°C before first use and 2−8°C after first use
resDNASEQ E1A DNA Real-Time PCR Reagents
• Environmental Master Mix (2X), 5 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light
• E1A DNA Short Fragment Assay Mix, 1 x 400 μL, store at –15 to –25°C, protect from light
• E1A DNA Medium Fragment Assay Mix, 1 x 660 μL, store at –15 to –25°C, protect from light
• E1A DNA Long Fragment Assay, 1 x 400 μL, store at –15 to –25°C, protect from light
• Negative Control (water), 3 x 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
PrepSEQ Nucleic Acid Extraction Kit
• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
• Elution Buffer (1 bottle, 25 mL)
• Proteinase K Buffer (1 bottle, 50 mL)
• Magnetic Particles (2 tubes, 1.5 mL/tube)
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL). Store at or below –20°C
• PK Buffer II (1 bottle, 11 mL)
Store all at room temperature unless otherwise indicated.
PrepSEQ Residual DNA Sample Preparation Kit
• Proteinase K (20 mg/mL), 1 tube, 1.25 mL
• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL
• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tube
Store all at or below –20°C.