Thermo Scientific™

TMTpro 18-plex, TMTpro-134C, and TMTpro-135N Label Reagents

Catalog number: A52047
Thermo Scientific™

TMTpro 18-plex, TMTpro-134C, and TMTpro-135N Label Reagents

Catalog number: A52047
TMTpro label reagents are the next generation of tandem mass tags, designed to increase the level of sample multiplexing without compromising on protein identification and quantitation. The Thermo Scientific TMTpro 18plex Label Reagent Set enables multiplexing up to 18 samples, enabling even greater throughput for protein identification and quantitative analysis by tandem mass spectrometry (MS).
 
Catalog Number
A52047
Unit Size
6 x 0.5 mg
Description
TMTpro™ 18-plex Label Reagent Set, 6 x 0.5 mg
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMTpro™ 18-plex Label Reagent Set, 6 x 0.5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity6 x 0.5 mg
Unit Size6 x 0.5 mg
Catalog Number
Unit Size
1 x 5 mg
Description
TMTpro™ 18-plex Label Reagent Set, 5 mg
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMTpro™ 18-plex Label Reagent Set, 5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity5 mg
Unit Size1 x 5 mg
Catalog Number
Unit Size
1 x 5 mg
Description
TMTpro-134C and TMTpro-135N Label Reagents, 5 mg
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMTpro-134C and TMTpro-135N Label Reagents, 5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity5 mg
Unit Size1 x 5 mg
Catalog Number
Unit Size
6 x 0.5 mg
Description
TMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity6 x 0.5 mg
Unit Size6 x 0.5 mg
Catalog Number
Unit Size
4 x 50 μg​ (per tag)
Description
TMTpro™ 18plex Label Reagents ​in stabilized solution, ​96-well plate, 4 x 50 μg​
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMTpro™ 18plex Label Reagents ​in stabilized solution, ​96-well plate, 4 x 50 μg​
FormulationLiquid in stabilized DMSO solution
For Use With (Equipment)Mass Spectrometer
Product TypeIsobaric Label Reagent Set
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Final Product TypeProtein PEPTIDE
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity72 Reactions
FormLiquid
Unit Size4 x 50 μg​ (per tag)
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
A52047Full specifications
6 x 0.5 mgTMTpro™ 18-plex Label Reagent Set, 6 x 0.5 mg
-
DescriptionTMTpro™ 18-plex Label Reagent Set, 6 x 0.5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity6 x 0.5 mg
Unit Size6 x 0.5 mg
A52045Full specifications
1 x 5 mgTMTpro™ 18-plex Label Reagent Set, 5 mg
-
DescriptionTMTpro™ 18-plex Label Reagent Set, 5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity5 mg
Unit Size1 x 5 mg
A52046Full specifications
1 x 5 mgTMTpro-134C and TMTpro-135N Label Reagents, 5 mg
-
DescriptionTMTpro-134C and TMTpro-135N Label Reagents, 5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity5 mg
Unit Size1 x 5 mg
A52048Full specifications
6 x 0.5 mgTMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg
-
DescriptionTMTpro-134C and TMTpro-135N Label Reagents, 6 x 0.5 mg
Product TypeIsobaric Label Reagent Set
Starting MaterialPeptides
Detection MethodMass Spectrometry
Workflow StepPeptide labeling and quantitation
Quantity6 x 0.5 mg
Unit Size6 x 0.5 mg
A58335Full specifications
4 x 50 μg​ (per tag)TMTpro™ 18plex Label Reagents ​in stabilized solution, ​96-well plate, 4 x 50 μg​
-
DescriptionTMTpro™ 18plex Label Reagents ​in stabilized solution, ​96-well plate, 4 x 50 μg​
FormulationLiquid in stabilized DMSO solution
For Use With (Equipment)Mass Spectrometer
Product TypeIsobaric Label Reagent Set
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Final Product TypeProtein PEPTIDE
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity72 Reactions
FormLiquid
Unit Size4 x 50 μg​ (per tag)
Showing 5 of 5

The structures of the TMTpro and TMT tags are similar in being isobaric and amine reactive, but the TMTpro tag has a longer spacer region and isobutyl proline mass reporter. After MS/MS fragmentation, each TMTpro 18plex tag generates a unique reporter mass (e.g., TMTpro 126-135 Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for relative quantitation of protein expression levels.

The TMT pro134C and TMTpro135N Label Reagents are identical in composition and structure to the other TMTpro 16plex tags, but are not isobaric. Addition of the TMTpro 134C and 135N tags create a 17th and 18th channel for relative quantitation using high resolution Orbitrap MS instruments. These tags are available separately (Cat. No. A52046, A52048), as part of the TMTpro 18plex Label Reagent Set, or as part of the complete 18plex set of reagents in a 96-well plate format.

Features of the TMTpro 18plex Label Reagent Set include:
Multiplex—concurrent MS analysis of up to 18 samples derived from cells, tissues, or biological fluids
Robust—increased multiplex capability results in fewer missing quantitative values among samples and higher confidence among replicates
Efficient—amine-reactive, NHS ester-activated reagents ensure greater than 95% labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
Optimized for use—with high resolution Thermo Scientific MS/MS Orbitrap platforms, analysis fully supported by Proteome Discoverer 2.3
Convenient size—provided in a ready-to-use, single-use format to ensure optimal stability and performance

For high resolution analysis of TMTpro-labeled peptides, the recommended LC column for the Nanospray Flex source is the Acclaim PepMap 100 C18 LC Column (Cat. No. 164942 or 164939). For the EASY-Spray source, the recommended LC column is the EASY-Spray C18 LC Column (Cat. No. ES902 or ES903). When combined with the industry-leading, high resolution Orbitrap instruments and software, TMTpro reagents provide an integrated total solution for quantitative protein expression analysis.

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