The eBioscience Human Lysed Whole Blood Cell Flow Cytometry Workflow Kit contains all the buffers, blockers, and beads to complete a standard flow cytometry experiment for staining cell surface targets on human lysed whole blood.
See protocol ›This kit contains:
• UltraComp eBeads Plus Compensation Beads (Cat. No. 01-3333-41)
• eBioscience 10X RBC Lysis Buffer (multi-species) (00-4300-54)
• Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience (14-9161-71)
• CellBlox Blocking Buffer (B001T06F01)
• eBioscience Flow Cytometry Staining Buffer (00-4222-57)
• eBioscience IC Fixation Buffer (00-8222-49)
When combined with the antibodies specific to your experiment, the reagents within this kit have been optimized to work together to detect cell surface targets by flow cytometry and broadly include:
• Effective compensation controls
• Fc receptor blocking
• Non-antibody, non-protein-based blocking solution for reducing background staining from fluorophores
• Antibody binding buffer that minimizes non-specific binding of antibodies
• Fixation buffer for storage of stained samples