How many genes and variant types are covered by the Oncomine Dx Express Test?
The Oncomine Dx Express Test (Cat. No. A54103) detects biomarkers across 46 unique genes. It detects substitutions, insertions, and deletions in 42 genes from DNA; copy number variants in 10 genes from DNA; and fusions or splice variants in 18 genes from RNA isolated from FFPE tumor samples.
Which PTEN gene alterations can the Oncomine Dx Express Test detect?
The Oncomine Dx Express Test (Cat. No. A54103) detects predefined PTEN substitutions, insertions, and deletions from DNA isolated from FFPE tumor samples. Refer to the Oncomine Dx Express Test User Guide for the complete list of validated PTEN variants.
How many DNA and RNA FFPE samples can I run with the Oncomine Dx Express Test?
Up to 14 FFPE DNA and RNA samples can be included in a sequencing run. The run also includes positive and no-template controls (NTCs) for both the DNA and RNA assays.
Which genes are included in the Oncomine Dx Express Test Panel?
The Oncomine Dx Express Test Panel is a targeted next-generation sequencing (NGS) assay that evaluates 46 genes across DNA and RNA. It detects multiple classes of genomic alterations, including single nucleotide variants (SNVs), insertions/deletions (indels), copy number variants (CNVs), gene fusions, and splice variants.
For the Oncomine Dx Express Test Panel, do the 32 reactions mean 16 DNA and 16 RNA reactions?
The 32 reactions can support up to 14 FFPE (DNA + RNA) samples or up to 20 plasma samples in the CE IVD version of ODxET. The FDA approved version does not support liquid biopsy and can be used for 14 FFPE samples. Some of the reactions are used for controls.
How many DNA and RNA samples can I analyze with one Oncomine Dx Express Test Panel kit?
The CE IVD version of Oncomine Dx Express Test Panel supports up to 14 FFPE (DNA + RNA) samples or up to 20 plasma samples. The FDA approved version does not support liquid biopsy and can be used for 14 FFPE samples.
What is the lowest DNA input that can be used with the Oncomine Dx Express Test Panel?
The Oncomine Dx Express Test has been validated using 10 ng of DNA and 10 ng of RNA per FFPE sample.