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TrueMark™ STI Select Panel Combo Kit

Applied Biosystems™

TrueMark™ STI Select Panel Combo Kit

The Applied Biosystems TrueMark STI Select Panel Combo Kit is a multiplex real‐time PCR solution that contains the assay and master mix needed for the simultaneous detection of RNA in extracted human samples.

Catalog Number	Quantity
A57083	200 reactions

Catalog number A57083

The Applied Biosystems TrueMark STI Select Panel Combo Kit is a multiplex real‐time PCR solution that contains the assay and master mix needed for the simultaneous detection of nucleic acid from Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Mycoplasma genitalium (MG), and Trichomonas vaginalis (TV) in extracted human samples. Human ribonuclease P (RNase P) is included as endogenous sample collection control.

Features of the TrueMark STI Select Panel Combo Kit include:
• Single test for CT, NG, MG, TV; detect and differentiate between clinically relevant targets found in sexually transmitted infections
• Affordable and scalable—add to existing infrastructure to expand menu while maintaining low operational costs
• Ready-to-use off-the-shelf solution—seamlessly integrate test into the existing workflow
• Research Use Only

The TrueMark STI Select Panel Combo Kit includes the TrueMark STI Select Panel and TrueMark Infectious Disease 1‐Step Multiplex Master Mix for simplified ordering. Together with the TrueMark STI Amplification Control, the kit can be used to quickly evaluate up to 200 samples and is validated for use with RNA extracted from vaginal swabs. The kit is recommended for use with the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit, the KingFisher Flex Purification System, and the QuantStudio 5 Real-Time PCR System (with 96-well, 0.2-mL block).

The TrueMark STI Select Panel Combo Kit includes the following components:
• TrueMark STI Select Panel—multiplexed assay that contains primer and probe sets specific to CT, NG, MG, TV, and RNase P targets
• TrueMark Infectious Disease 1‐Step Multiplex Master Mix (No ROX)

Use this kit with:
• TrueMark STI Amplification Control (Cat. No. A57009)—plasmid control containing templates specific to CT, NG, MG, TV, and RNase P

For Use With (Equipment)QuantStudio™ 5 Real‐Time PCR System, 96-well, 0.2-mL block

No. of Reactions200 Reactions

Quantity200 reactions

Detection MethodPrimer-probe

For Use With (Application)Pathogen Detection

Frequently asked questions (FAQs)

Can I order the assays in the TrueMark STI Select Panel Combo Kit (Cat. No. A57083) separately?

No, the assay IDs for the individual target assays in the TrueMark STI Select Panel are not provided separately.

Do I need to use RNase P with the assays included in the TrueMark, STI Select Panel, Combo Kit?

Yes, RNase P is the human internal sample control target utilized in the TrueMark, STI Select Panel, Combo Kit and it is one of the multiplexed assays in the kit.

Which instruments, consumables, and reagents do I need for the TrueMark STI Select Panel, Combo Kit? Where can I find the full list of products and protocols?

We recommend running the TrueMark STI Select Panel, Combo Kit on the QuantStudio 5 Real-Time PCR instrument, 96-well, 0.2 mL block though other instruments that have the ability to detect Alexa Fluor 647 dye are capable of running the kit. Information about other reagents and consumables that are needed can be found in the user guide ((https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0028493-TrueMarkSTISelectPanel-UG-RUO-EN.pdf).

Do you provide run templates for the TrueMark STI Select Panel, Combo Kit?

No, these are single-tube assays and the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0028493-TrueMarkSTISelectPanel-UG-RUO-EN.pdf) provides details on how to setup and create a template to detect and assign the targets to each well.

What are the components of the TrueMark STI Select Panel, Combo Kit? Are there any other required materials that are not supplied?

The TrueMark STI Select Panel, Combo Kit (Cat. No. A57083) contains the TrueMark STI Select Panel (Part No. A52691) and the TrueMark Infectious Disease 1-Step Multiplex Master Mix (No ROX) (Part No. A57052). Other required materials are standard qPCR consumables and instruments and they can be found in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0028493-TrueMarkSTISelectPanel-UG-RUO-EN.pdf).