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Invitrogen
Carboxypeptidase Y Monoclonal Antibody (10A5B5)
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FIGURE: 1 / 19
Carboxypeptidase Y Antibody (A-6428) in WB
Cells 2024 -
Figure 4 VPS27 deletion impairs the CPY pathway in the sit4 Delta mutant. ( A ) Immunoblotting showing complete processing of CPY in both Log and PDS (24 h after Log) phases in all strains (except in pep4 Delta cells, used as control). ( B ) CPY protein levels were quantified using GAPDH as a loading control. Values are the mean +- SD ( n = 4). ( C ) CPY secretion was assessed using a colony immunoblot assa... View More
Product Details
A-6428
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Published species
Yeast
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
10A5B5
Immunogen
Purified yeast CPY (carboxypeptidase)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Ig fraction
Storage buffer
HEPES buffered saline
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.
Target Information
Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; cpy1; pcy1
Gene Aliases: CPY1; LBC1; PRC1; YMR297W
UniProt ID: (Baker's yeast) P00729
Entrez Gene ID: (Baker's yeast) 855343
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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