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Verso 1-step RT-qPCR Mix, ROX, 200 x 25 μL Reactions - FAQs

Verso 1-step RT-qPCR Mix, ROX, 200 x 25 μL Reactions - FAQs

View additional product information for Verso 1-step RT-qPCR Mix, ROX - FAQs (AB4101A, AB4101C)

2 product FAQs found

What is the RT Enhancer that is included in all Verso kits?

The RT Enhancer is a unique enzyme that degrades contaminating genomic DNA at the start of the RT step. It is equally as effective as DNase I treatment but more convenient as it eliminates the need for a separate DNase I incubation prior to RT-PCR.

Do I need to treat my RNA with DNase I before running RT-qPCR with Verso cDNA Synthesis Kit?

No, you do not need to treat your RNA with DNase I before running RT-qPCR with the Verso cDNA Synthesis Kit. The RT enhancer included in the Verso kit degrades any contaminating double-stranded DNA during the reverse transcription process, eliminating the need for a separate DNase I treatment.