The Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Features of the Verso 1-step RT-qPCR ROX Mix Kit:
• Eliminate the need for DNase treatments—RT Enhancer (optional) prevents gDNA carry-over • RT and qPCR steps performed in single tube—convenience and reduced contamination risk • Use of the entire RT reaction as template for qPCR—improved sensitivity • Verso RT enzyme is highly sensitive with broad dynamic range—accurate detection of RNA from 1 pg-1 ug • Verso RT enzyme has a wide working temperature range (42 to 57°C)—improved success with GC-rich and other difficult templates • Contains an inert blue dye for ease of pipetting during reaction set up
An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.
In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments. The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling.