ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Catalog number: AB4166B

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.


High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes


• Gene expression analysis
• siRNA validation
• Genotyping

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For Research Use Only. Not for use in diagnostic procedures.


Detection Method: SYBR
For Use With (Equipment): Cepheid SmartCycler, Eppendorf MasterCycler RealPlex, Roche LightCycler 480, Bio-Rad CFX384 Touch™, BioRad CFX96™, Corbett RotorGene, MJ Chromo4, MJ Opticon
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): dsDNA, DNA
Polymerase: Taq DNA Polymerase
Product Size: 1,600 reactions
Number of Reactions: 1,600 Reactions

Contents & storage

• 16 x 1.25 mL ABsolute Blue QPCR SYBR Green Mix (2X)
• 100 µL MgCl2 (1M)
• 25 µL ROX Reference Dye (1 mM)
Sufficient for 1600 x 25 µL reactions.

Store at -20°C


Manuals & protocols

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