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Invitrogen
TNF alpha Antibody Cocktail
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FIGURE: 1 / 1
TNF alpha Antibody (AHC3712) in ELISA
The Journal of experimental medicine 1999 -
Figure 3 CT treatment selectively suppressed IL-12 p70 and TNF-alpha, but not IL-10, TGF-beta 1 , or IL-6. Human monocytes (2 x 10 6 cell/mL) were stimulated with SAC (0.01%) and IFN-gamma (100 ng/mL) in the presence or absence of CT (10 ng/mL). Cytokine production was measured by ELISA 24 h after stimulation. Data are mean values +- SD of four separate experiments using four different donors. ** P <... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
AHC3712
Applications
Tested Dilution
Publications
Product Specifications
Published species
Bacteria,
Human
Host/Isotype
Mouse
/ Ig Mix
Class
Cocktail
Type
Antibody
Clone
68B2B3 and 68B6A3
Immunogen
Purified recombinant human TNF-alpha.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
500 µg
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This monoclonal mixture is recommended as a capture antibody in Sandwich ELISA applications.
This clone can also be referred to as clone number 68B 2B3 BC6/68B 6A3 1H8 1F2.
Clone 68B2B3 isotype is undetermined. Clone 68B6A3 isotype is IgG1.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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