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          • Primary Antibodies ›
          • mTOR Antibodies

          Invitrogen

          mTOR Monoclonal Antibody (215Q18)

          Advanced Verification
          13 Published Figures
          16 References
          View all (106) mTOR antibodies

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          Cite mTOR Monoclonal Antibody (215Q18)

          Additional Information:
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          • Antibody Testing Data (4)
          • Published Figures (13)
          • Advanced Verification (1)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
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          mTOR Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 18

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          mTOR Antibody (AHO1232) in ICC/IF

          Immunofluorescent analysis of mTOR was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with mTOR Mouse monoclonal Antibody (Product # AHO1232) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at roo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Immunocytochemistry (ICC/IF)
          mTOR Antibody in Immunohistochemistry (IHC)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot (WB)
          mTOR Antibody in Western Blot, Immunoprecipitation (WB, IP)
          mTOR Antibody

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          AHO1232

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          Assay-dependent
          View 10 publications 10 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:10-1:100
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          215Q18

          Immunogen

          Recombinant fragment of human mTOR expressed in E. coli.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536329

          Target Information

          FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; m-TOR; mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mTORC1; rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; tyrosine-protein kinase mTOR; unnamed protein product

          View more View less

          Gene Aliases: 2610315D21Rik; flat; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS

          View more View less

          UniProt ID: (Human) P42345, (Rat) P42346, (Mouse) Q9JLN9

          View more View less

          Entrez Gene ID: (Human) 2475, (Rat) 56718, (Mouse) 56717

          View more View less

          Function(s)
          inositol hexakisphosphate binding RNA polymerase III type 1 promoter sequence-specific DNA binding RNA polymerase III type 2 promoter sequence-specific DNA binding RNA polymerase III type 3 promoter sequence-specific DNA binding TFIIIC-class transcription factor binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding kinase activity identical protein binding ribosome binding ion channel binding macromolecular complex binding phosphoprotein binding protein serine kinase activity protein kinase binding protein domain specific binding
          Process(es)
          regulation of cell growth T-helper 1 cell lineage commitment 'de novo' pyrimidine nucleobase biosynthetic process protein phosphorylation inflammatory response cellular response to DNA damage stimulus cytoskeleton organization regulation of cell size cellular response to starvation response to heat regulation of autophagy negative regulation of autophagy positive regulation of epithelial to mesenchymal transition regulation of macroautophagy negative regulation of macroautophagy regulation of lipid metabolic process neuronal action potential positive regulation of cell growth T cell costimulation response to nutrient levels cellular response to nutrient levels cellular response to nutrient TOR signaling cellular response to insulin stimulus regulation of actin cytoskeleton organization cellular response to amino acid starvation TORC1 signaling TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process response to amino acid anoikis protein kinase B signaling positive regulation of cell differentiation regulation of osteoclast differentiation positive regulation of translation positive regulation of glycolytic process positive regulation of protein kinase activity positive regulation of transcription from RNA polymerase III promoter positive regulation of translational initiation negative regulation of insulin receptor signaling pathway positive regulation of lipid biosynthetic process behavioral response to pain protein stabilization positive regulation of multicellular organismal process positive regulation of keratinocyte migration regulation of protein kinase B signaling positive regulation of protein kinase B signaling cardiac cell development cellular response to methionine positive regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process cellular response to amino acid stimulus cellular response to leucine cellular response to hypoxia cellular response to osmotic stress regulation of cellular response to stress vascular endothelial cell response to laminar fluid shear stress regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of wound healing, spreading of epidermal cells regulation of locomotor rhythm positive regulation of cytoplasmic translational initiation regulation of lysosome organization negative regulation of lysosome organization positive regulation of pentose-phosphate shunt cellular response to leucine starvation positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of autophagosome assembly regulation of protein phosphorylation negative regulation of protein phosphorylation positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation heart morphogenesis heart valve morphogenesis regulation of glycogen biosynthetic process regulation of carbohydrate metabolic process energy reserve metabolic process germ cell development long-term memory visual learning response to virus post-embryonic development positive regulation of lamellipodium assembly positive regulation of myotube differentiation positive regulation of neuron projection development positive regulation of neuron maturation negative regulation of muscle atrophy response to activity peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization positive regulation of actin filament polymerization positive regulation of cell projection organization negative regulation of protein ubiquitination regulation of myelination regulation of fatty acid beta-oxidation regulation of response to food response to insulin social behavior multicellular organism growth response to cocaine positive regulation of neuron apoptotic process regulation of carbohydrate utilization positive regulation of nitric oxide biosynthetic process negative regulation of cell size regulation of protein kinase activity protein autophosphorylation mRNA stabilization positive regulation of smooth muscle cell proliferation positive regulation of oligodendrocyte differentiation cardiac muscle tissue development positive regulation of peptidyl-tyrosine phosphorylation positive regulation of neurogenesis regulation of behavior voluntary musculoskeletal movement positive regulation of striated muscle cell differentiation positive regulation of stress fiber assembly cardiac muscle cell development cardiac muscle contraction maternal process involved in female pregnancy positive regulation of glial cell proliferation positive regulation of dendritic spine development positive regulation of cell growth involved in cardiac muscle cell development negative regulation of calcineurin-NFAT signaling cascade regulation of brown fat cell differentiation regulation of membrane permeability regulation of translation at synapse, modulating synaptic transmission regulation of plasma membrane bounded cell projection organization positive regulation of ruffle assembly positive regulation of eating behavior positive regulation of epithelial cell apoptotic process positive regulation of cholangiocyte proliferation negative regulation of cholangiocyte apoptotic process positive regulation of granulosa cell proliferation positive regulation of skeletal muscle hypertrophy negative regulation of iodide transmembrane transport positive regulation of cellular senescence autophagosome assembly cytoplasmic translation regulation of translational initiation ubiquitin-dependent protein catabolic process lysosome organization positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of autophagy SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization negative regulation of innate immune response negative regulation of translational initiation antibacterial innate immune response negative regulation of TORC2 signaling negative regulation of TORC1 signaling positive regulation of TORC1 signaling negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.

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