Applied Biosystems™

AgPath-ID™ One-Step RT-PCR Reagents

Catalog number:  AM1005

 Related applications:

Animal Health

| Real Time PCR (qPCR)

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AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan® real-time reverse transcription PCR (RT-PCR) strategy.
• Recommended for RNA pathogen amplification
• Consistent amplification of RNA targets with high specificity and sensitivity
• Optimized to work with your target-specific primers and probes
• Contains ROX for quantitative fluorescent signal normalization

AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold® polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


PCR Method: 1-Step qRT-PCR
Reverse Transcriptase: ArrayScript™
Polymerase: AmpliTaq Gold DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
For Use With (Equipment): 7500 System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Comments: Works on all AB platforms, but is optimized for the 7500.
Product Line: AgPath-ID™, Ambion™
Product Size: 100 reactions
Shipping Condition: Dry Ice

Contents & storage

Sufficient for 100 reactions of 25 µL, contains:
• 1375µL 2X RT-PCR Buffer
• 110µL 25X RT-PCR Enzyme Mix
• 190µL Detection Enhancer
• 1.75mL Nuclease-free Water.


Manuals & protocols