mMESSAGE mMACHINE™ SP6 Transcription Kit - FAQs

What is the control included in the mMESSAGE mMACHINE kit?

The control is a pTRI-Xef control template which is a linearized TRIPLEscript plasmid contianing the 1.8 kb Xenopus elongation factor 1alpha gene under the transcription control of tandem SP6, T7, and T3 promoters (pTRI-Xef 1). Any one of the three polymerases can be used to synthesize the control RNA.