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mirVana™ miRNA Isolation Kit, with phenol

Catalog number: AM1560

mirVana™ miRNA Isolation Kit, with phenol

Catalog number: AM1560
Catalog Number
Unit Size
40 preps
Price (USD)
Price: 469.00
Online price: 431.65
Your price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM156040 preps
Price: 469.00
Online price: 431.65
Your price:
Product Overview
Citations & References
Additional Information
The mirVana miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.

Features of this kit include:

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107 cultured cells or 0.5–250 mg tissue.

Enrich for small RNA
Although the mirVana miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

Accessory product
The mirVana miRNA Isolation Kit without Phenol is available for customers concerned about the cost of shipping organic chemicals such as Acid Phenol:Chloroform, which is included in the standard kit.
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


Isolation Technology
Spin Column (Glass Fiber Filter), Organic Extraction
Purification Time
30 min. (After sample homogenization)
Elution Volume
100 μL
Sample Type
Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
Final Product Type
Small RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
qRT-PCR, microarray analysis, Northern blotting
40 Preps
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤107 cells
Cells: ≤107
Plant: ≤250 mg
Tissue: ≤250 mg
Virus: ≤107 cells
Yeast: ≤107 cells
Enzymatic Reactions: varies
Cells: ≤16 μg RNA per 50,000 cells
Tissue: ≤160 μg per 5 mg

Contents & Storage

• 30 mL miRNA Wash Solution I (room temperature)
• 50 mL Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 mL Lysis/Binding Buffer (4°C)
• 10 mL miRNA Homogenate Additive (4°C)
• 100 mL Acid-Phenol:Chloroform (4°C)
• 1.4 mL Gel Loading Buffer II (-20°C)
• 5 mL Elution Solution (-20°C, 4°C, or room temperature)


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    Frequently asked questions (FAQs)

    Citations & References

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