Invitrogen™

mirVana™ miRNA Isolation Kit, with phenol

Catalog number:  AM1560

Related applications:

RNAi

| RNA Extraction

| miRNA & Non-Coding RNA Analysis

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Description

The mirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions.

Features of this kit include:

• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue types

Efficient recovery of small RNA
Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. The mirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. The mirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107 cultured cells or 0.5–250 mg tissue.

Enrich for small RNA
Although the mirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in the mirVana™ miRNA Isolation Kit, RNA molecules of ~200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using the mirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.

Accessory product
The mirVana™ miRNA Isolation Kit without Phenol is available for customers concerned about the cost of shipping organic chemicals such as Acid Phenol:Chloroform, which is included in the standard kit.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: Total RNA, Transcriptome RNA, siRNA, snRNA, micro RNA
For Use With (Application): microRNA Analysis
Purification Target: siRNA, miRNA
Sample Type: Plant Samples, Viral Samples, Tissue, Yeast, Bacteria, Cells
Includes: 30mL miRNA Wash Solution I; 50mL Wash Solution 2/3; 80 Collection Tubes; 40 Filter Cartridges; 100mL Lysis/Binding Buffer; 10mL miRNA Homogenate Additive; 100mL Acid-Phenol:Chloroform; 1.4mL Gel Loading Buffer II; 5mL Elution Solution
Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Starting Material Amount: Up to 250 mg tissue, Up to 10^7 cells
Product Line: mirVana™, Ambion™

Contents & storage

• 30 ml miRNA Wash Solution I (room temperature)
• 50 ml Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 100 ml Lysis/Binding Buffer (4°C)
• 10 ml miRNA Homogenate Additive (4°C)
• 100 ml Acid-Phenol:Chloroform (4°C)
• 1.4 ml Gel Loading Buffer II (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, or room temperature)