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View additional product information for LeukoLOCK™ Total RNA Isolation System - FAQs (AM1923)
7 product FAQs found
Yes. You will need to follow the alternate protocol, available in the following link.
We have processed blood stored with heparin with the LeukoLOCK Total RNA Isolation System. The RNA looks fine. But we have not tested it for any downstream applications. We discourage your use of heparin, as heparin notoriously inhibits downstream applications such as RT-PCR.
No, they are based on different chemistries.
The LeukoLOCK Total RNA Isolation System has been tested only for human blood.
No. The kit has not been validated for less than 3 mL of human blood. It is best to use 9-10 mL blood.
RNA yield is typically 10-20 µg from 10 mL of blood. RNA quality depends on the quality of the blood. Typically, the RNA integrity number (RIN) value is around 9, and the 28S/18S ratio is around 1.5.
RNA recovered using the LeukoLOCK Total RNA Isolation System typically contains less than 10% of the amount of reticulocyte-derived alpha- and beta-globin mRNAs present in typical mRNA samples from unfractionated whole blood. The isolated RNA is specially suited for gene expression profiling, as it contains a lower proportion of globin mRNA. We sell LeukoLOCK Fractionation and Stabilization Kit (Cat. No. AM1933) for blood collection and stabilization. You can then use LeukoLOCK Total RNA Isolation Kit (Cat. No. AM1934) to isolate RNA stabilized on the LeukoLOCK filters. Cat. Nos. AM1934 and AM1933 are available together, packaged in a single kit as the LeukoLOCK Total RNA Isolation System (Cat. No. AM1923).