RiboPure™ RNA Purification Kit, blood - FAQs

View additional product information for RiboPure™ RNA Purification Kit, blood - FAQs (AM1928)

6 product FAQs found

What is the difference between the RiboPure Blood Purification Kit (Cat. No. AM1928) and Mouse RiboPure Blood RNA Isolation kit (Cat. No. AM1951)?

The former (Cat. No. AM1928) is for isolation of RNA from human blood and the latter (Cat. No. AM1951) is for isolation of RNA from mouse and rat blood. Both kits have the same columns but differ in protocol and the wash 1 buffer, wash 2/3 buffer, and sodium acetate solutions. We do not recommend using the mouse kit for human blood samples. The mouse kit accommodates the difference in viscosity of mouse/rat vs. human blood and also the higher mouse and rat blood RNA yield. The mouse kit allows recovery of either total RNA containing small RNAs or total RNA depleted of small RNAs based on which protocol in the manual was followed. With the RiboPure Blood Purification Kit, using the standard protocol in the manual, you can isolate total RNA depleted of small RNAs, but with an alternate protocol, you can isolate total RNA containing small RNAs. Alternatively, if you need small RNAs alone, then you should follow the above alternate protocol for isolation of total RNAs containing small RNAs, and then follow Section IVA in the mirVana miRNA Isolation Kit manual (Cat. No AM1560).

What is the composition of the elution solution in the RiboPure-Blood Purification Kit (Cat. No. AM1928)?

Its composition is 0.1 mM EDTA in nuclease-free water.

What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Do you have any tips for RNA isolation when working with blood samples?

Please visit our website for tips for working with blood samples.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

Can I use the RiboPure RNA Purification Kit, blood (Cat. No. AM1928) to extract RNA from blood samples?

For RNA purification from blood samples, in RNAlater, the RiboPure RNA Purification Kit, blood is recommended.
Please note the ratio of blood to RNAlater should be 300-500 µL blood for 1.3 mL of RNAlater.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.