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Invitrogen™

Ambion™ RNase I, cloned, 100 U/μL

Catalog number: AM2295
Have Questions?
Invitrogen™

Ambion™ RNase I, cloned, 100 U/μL

Catalog number: AM2295
Have Questions?
Catalog Number
AM2295
Unit Size
25,000 units
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
10,000 units
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM229525,000 units
-
AM229410,000 units
-
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Ambion™ RNase I efficiently cleaves after all four bases of single-stranded RNA, in contrast to RNase A, which only cleaves after C and U residues. RNase I degrades all RNA dinucleotide bonds leaving a 5' hydroxyl and 2', 3' cyclic monophosphate. Supplied in one tube of 25,000 U (100 U/ μL). RNase I will degrade any RNA to a mixture of mononucleotides, dinucleotides, and trinucleotides and does not degrade DNA, although it will bind to DNA. It has a marked preference for single-stranded RNA over double-stranded RNA, which allows it to work well in ribonuclease protection assays. This RNase I is rigorously tested for nonspecific contaminating endonuclease, exonuclease, and protease activity.

Unit Definition:
One unit is the amount of enzyme required to produce 1 μg of acid-soluble material from mouse liver RNA in 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
100 U/μL
Shipping Condition
Dry Ice
Enzyme
RNase
Quantity
25,000 U
Product Type
RNase I
Product Line
Ambion™

Contents & Storage

-20°C

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