pMIR-REPORT™ miRNA Expression Reporter Vector System

Catalog number: AM5795

Invitrogen™  Related applications: RNAi | miRNA & Non-Coding RNA Analysis

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Description

The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

Applications:
pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: CMV
Reporter: Beta-Gal (lacZ), Luciferase
RNAi Type: miRNA
Vector Type: pMIR
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Delivery Method: Transfection
Selection Marker Promoter: SV40 Promoter
Selection Agent (Eukaryotic): Puromycin
Constitutive or Inducible System: Constitutive
Shipping Condition: Dry Ice

Contents & storage

pMIR-REPORT™ Luciferase and pMIR-REPORT™ β-gal Control Plasmid are both supplied as glycerol stocks in E. coli and should be stored at –70°C.

Documents

Manuals & protocols