pMIR-REPORT™ miRNA Expression Reporter Vector System

Catalog number:  AM5795

 Related applications:


| miRNA & Non-Coding RNA Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

• Clone miRNA targets and evaluate miRNA regulation
• Screen putative miRNA target sequences
• Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.
For Research Use Only. Not for use in diagnostic procedures.


Cloning Method: Restriction Enzyme ⁄ MCS
Constitutive or Inducible System: Constitutive
Delivery Method: Transfection
Product Line: Ambion™, pMIR-REPORT™
Product Size: 1 kit
Promoter: CMV
RNAi Type: miRNA
Reporter: Beta-Gal (lacZ), Luciferase
Selection Agent (Eukaryotic): Puromycin
Selection Marker Promoter: SV40 Promoter
Vector Type: pMIR
Shipping Condition: Dry Ice

Contents & storage

pMIR-REPORT™ Luciferase and pMIR-REPORT™ β-gal Control Plasmid are both supplied as glycerol stocks in E. coli and should be stored at –70°C.


Manuals & protocols