Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.
Use the right buffer to optimize protein separations Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.