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Invitrogen™

BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers

Catalog number:  B1151

Related applications:

Cell Viability, Proliferation & Function

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Description

BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Microplate Reader
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Indicator Properties: Fluorescent Dye-Based
Indicator: pH Indicator
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.