BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers

BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pH indicator with a pK_a of ∼6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm.

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