ABA-regulated promoter activity in stomatal guard cells.
AuthorsTaylor JE, Renwick KF, Webb AA, McAinsh MR, Furini A, Bartels D, Quatrano RS, Marcotte WR, Hetherington AM
JournalPlant J
PubMed ID7894503
'CDeT6-19 is an ABA-regulated gene which has been isolated from Craterostigma plantagineum. The CDeT6-19 gene promoter has been fused to the beta-glucuronidase reporter gene (GUS) and used to stably transform Arabidopsis thaliana and Nicotiana tabacum. This construct has been shown to be expressed in stomatal guard cells and often in ... More
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
AuthorsJefferson RA, Kavanagh TA, Bevan MW
JournalEMBO J
PubMed ID3327686
'We have used the Escherichia coli beta-glucuronidase gene (GUS) as a gene fusion marker for analysis of gene expression in transformed plants. Higher plants tested lack intrinsic beta-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. We have constructed gene fusions using the cauliflower mosaic virus (CaMV) ... More
Methods for Escherichia coli identification in food, water and clinical samples based on beta-glucuronidase detection.
AuthorsFrampton EW, Restaino L
JournalJ Appl Bacteriol
PubMed ID8468256
Genetic transformation of cassava (Manihot esculenta Crantz).
AuthorsLi HQ, Sautter C, Potrykus I, Puonti-Kaerlas J
JournalNat Biotechnol
PubMed ID9630981
'Genetic engineering can be used to complement traditional breeding methods in crop plant improvement. Transfer of genes from heterologous species provides the means of selectively introducing new traits into crop plants and expanding the gene pool beyond what has been available to traditional breeding systems. The prerequisites for genetic engineering ... More
Regulation of beta-glucuronidase expression in transgenic tobacco plants by an A/T-rich, cis-acting sequence found upstream of a French bean beta-phaseolin gene.
AuthorsBustos MM, Guiltinan MJ, Jordano J, Begum D, Kalkan FA, Hall TC
JournalPlant Cell
PubMed ID2535526
'A 0.8-kilobase fragment from the 5''-flanking region of a French bean beta-phaseolin gene yielded strong, temporally regulated, and embryo-specific expression of beta-glucuronidase (GUS) in transgenic tobacco plants, paralleling that found for the seed protein phaseolin [Sengupta-Gopalan, C., Reichert, N.A., Barker, R.F., Hall. T.C., and Kemp, J.D. (1985) Proc. Natl. Acad. ... More
Histochemical analysis of CaMV 35S promoter-beta-glucuronidase gene expression in transgenic rice plants.
AuthorsBattraw MJ, Hall TC
JournalPlant Mol Biol
PubMed ID2102372
'The cauliflower mosaic virus promoter is commonly used to drive transcription of chimeric genes in transgenic plants, including the cereals. To determine the tissue and cell types of cereal plants that the promoter functions in, transgenic rice plants containing a CaMV 35S promoter/GUS chimeric gene were analyzed for GUS activity. ... More
Protein stabilization by engineered metal chelation.
AuthorsKellis JT, Todd RJ, Arnold FH
JournalBiotechnology (N Y)
PubMed ID1369354
'A ligand can shift a protein''s folding/unfolding equilibrium by binding with higher affinity to the native state. A metal-chelating site consisting of two histidines separated by three residues (His-X3-His) engineered into an alpha-helix provides a general and easily-implemented means for protein stabilization by this mechanism. We have tested this approach ... More
Protein microchips: use for immunoassay and enzymatic reactions.
AuthorsArenkov P, Kukhtin A, Gemmell A, Voloshchuk S, Chupeeva V, Mirzabekov A
JournalAnal Biochem
PubMed ID10660453
'Different proteins such as antibodies, antigens, and enzymes were immobilized within the 100 x 100 x 20-microm gel pads of protein microchips. A modified polyacrylamide gel has been developed to accommodate proteins of a size up to 400,000 daltons. Electrophoresis in the microchip reaction chamber speeded up antigen-antibody interactions within ... More
AuthorsLorincz M, Roederer M, Diwu Z, Herzenberg LA, Nolan GP
JournalCytometry
PubMed ID8866216
'We report the development of a new fluorescence-activated cell sorter (FACS)-based reporter gene system utilizing the enzymatic activity of the E. coli beta-glucuronidase (gus) gene. When loaded with the Gus substrate fluorescein-di-beta-D-glucuronide (FDGlcu), individual mammalian cells expressing and translating gus mRNA liberate sufficient levels of intracellular fluorescein for quantitative analysis ... More
E. coli beta-glucuronidase (GUS) as a marker for recombinant vaccinia viruses.
AuthorsCarroll MW, Moss B
JournalBiotechniques
PubMed ID7495543
Localizing distribution of beta-glucuronidase in individual cells.
AuthorsSittertz-Bhatkar H
JournalBiotechniques
PubMed ID2698666
Improved beta-glucuronidase reporter for mammalian gene expression analysis.
AuthorsChan S, Plumb J, Hu J
JournalBiotechniques
PubMed ID10572632
Reporter genes and transient assays for plants.
AuthorsMatthews BF, Saunders JA, Gebhardt JS, Lin JJ, Koehler SM
JournalMethods Mol Biol
PubMed ID8528416
Reporter genes.
AuthorsSuter-Crazzolara C, Klemm M, Reiss B
JournalMethods Cell Biol
PubMed ID8531814
The GUS reporter gene system.
AuthorsJefferson RA
JournalNature
PubMed ID2689886
The GUS reporter gene system is already a powerful tool for the assessment of gene activity in transgenic plants. Further developments may lead to routine in vivo analysis and fusion genetics. ... More
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.
AuthorsIshida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T
JournalNat Biotechnol
PubMed ID9630983
Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried "super-binary" vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the ... More
AtPIN4 mediates sink-driven auxin gradients and root patterning in Arabidopsis.
AuthorsFriml J, Benková E, Blilou I, Wisniewska J, Hamann T, Ljung K, Woody S, Sandberg G, Scheres B, Jürgens G, Palme K
JournalCell
PubMed ID11893337
In contrast to animals, little is known about pattern formation in plants. Physiological and genetic data suggest the involvement of the phytohormone auxin in this process. Here, we characterize a novel member of the PIN family of putative auxin efflux carriers, Arabidopsis PIN4, that is localized in developing and mature ... More
Gene cloning in plants: innovative approaches.
AuthorsSawahel W, Fukui K
JournalBiotechniques
PubMed ID7669284
Although many technical advances have recently been made in the development of plant transformation systems and plant-gene identification and isolation approaches, some of these approaches are either technically or practically not feasible for many important gene systems; thus alternative approaches are required. Two innovative approaches for gene cloning in plants ... More
Direct influence of S9 liver homogenate on fluorescence signals: impact on practical applications in a bacterial genotoxicity assay.
AuthorsDreier J, Breitmaier EB, Gocke E, Apfel CM, Page MG
JournalMutat Res
PubMed ID11719102
Assays based on the bacterial SOS-response offer the possibility of automatization of genotoxicity testing for screening of large compound libraries. While existing assays use colorimetric detection or luminescence read-out, we describe here the use of a fluorescence-based system to achieve high sensitivity of detection required for assay miniaturization. Three commonly ... More
Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures.
AuthorsSchöpke C, Taylor N, Cárcamo R, Konan NK, Marmey P, Henshaw GG, Beachy RN, Fauquet C
JournalNat Biotechnol
PubMed ID9630980
A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene encoding neomycin phosphotransferase (nptII) and ... More
Inheritance and expression of chimeric genes in the progeny of transgenic maize plants.
AuthorsFromm ME, Morrish F, Armstrong C, Williams R, Thomas J, Klein TM
JournalBiotechnology (N Y)
PubMed ID1366794
We obtained transgenic maize plants by using high-velocity microprojectiles to transfer genes into embryongenic cells. Two selectable genes were used to confer resistance to either chlorsulfuron or phosphinothricin, and genes encoding either E. coli beta-glucuronidase or firefly luciferase were used as markers to provide convenient assays for transformation. When regenerated ... More
beta-Glucuronidase from Escherichia coli as a gene-fusion marker.
AuthorsJefferson RA, Burgess SM, Hirsh D
JournalProc Natl Acad Sci U S A
PubMed ID3534890
We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide sequence has been determined. beta-Glucuronidase has been purified to homogeneity and characterized. The enzyme has a subunit molecular weight of 68,200, is ... More
T-DNA transfer to maize cells: histochemical investigation of beta-glucuronidase activity in maize tissues.
AuthorsShen WH, Escudero J, Schläppi M, Ramos C, Hohn B, Koukolíková-Nicola Z
JournalProc Natl Acad Sci U S A
PubMed ID11607370
Agrobacterium tumefaciens is routinely used to engineer desirable genes into dicotyledonous plants. However, the economically important graminaceous plant maize is refractory to tumor induction by inoculation with virulent strains of A. tumefaciens. Currently, the only clearcut evidence for transferred DNA (T-DNA) transport from Agrobacterium to maize comes from agroinfection. To ... More
Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident.
AuthorsKovalchuk I, Kovalchuk O, Arkhipov A, Hohn B
JournalNat Biotechnol
PubMed ID9831035
To evaluate the genetic consequences of radioactive contamination originating from the nuclear reactor accident of Chernobyl on indigenous populations of plants and animals, it is essential to determine the rates of accumulating genetic changes in chronically irradiated populations. An increase in germline mutation rates in humans living close to the ... More
GT-1 binding site confers light responsive expression in transgenic tobacco.
AuthorsLam E, Chua NH
JournalScience
PubMed ID2330508
Light-dependent expression of rbcS, the gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase, which is the key enzyme involved in carbon fixation in higher plants, is regulated at the transcriptional level. Sequence analysis of the gene has uncovered a conserved GT motif in the -150 to -100 region of many ... More
Non-autonomous regulation of a graded, PKA-mediated transcriptional activation signal for cell patterning.
AuthorsBalint-Kurti P, Ginsburg GT, Liu J, Kimmel AR
JournalDevelopment
PubMed ID9735356
The pseudoplasmodium or migrating slug of Dictyostelium is composed of non-terminally differentiated cells, organized along an anteroposterior axis. Cells in the anterior region of the slug define the prestalk compartment, whereas most of the posterior zone consists of prespore cells. We now present evidence that the cAMP-dependent protein kinase (PKA) ... More