DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
DiSBAC<sub>2</sub>(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)
Citas y referencias (120)
Invitrogen™

DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol)

La sonda sensible al potencial de respuesta lenta, DiSBAC2(3) puede entrar en células despolarizadas donde se une a las proteínasMás información
Have Questions?
Número de catálogoCantidad
B413100 mg
Número de catálogo B413
Precio (USD)
536,22
Each
Añadir al carro de la compra
Cantidad:
100 mg
Precio (USD)
536,22
Each
Añadir al carro de la compra
La sonda sensible al potencial de respuesta lenta, DiSBAC2(3) puede entrar en células despolarizadas donde se une a las proteínas intracelulares o a la membrana y muestra una mayor fluorescencia y un cambio espectral rojo. El aumento de la despolarización produce un flujo adicional del colorante aniónico y un aumento de la fluorescencia. Por el contrario, la hiperpolarización se indica mediante una disminución de la fluorescencia. Los cambios de fluorescencia dependientes del potencial generados por este bis-oxonol son típicamente ∼ 1 % por mV. Además, esta sonda tiene un máximo de excitación de 530 nm y un máximo de emisión de 560 nm. Los colorantes DiBAC se excluyen de las mitocondrias debido a su carga negativa general, haciéndolos superiores a las carbocianinas para medir los potenciales de la membrana plasmática.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de coloranteSondas de potencial de membrana
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Tipo de productoDiSBAC2(3)
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.

Preguntas frecuentes

I am seeing high background outside of my neuronal cells when using membrane potential indicators. What can I do to reduce background?

If you use our FluoVolt Membrane Potential Kit (Cat. No. F10488), the kit provides a background suppressor to reduce this problem. For other indicators, consider the use of BackDrop Background Suppressor (Cat no. R37603, B10511, and B10512).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the difference between fast and slow-response membrane potential probes?

Molecules that change their structure in response to the surrounding electric field can function as fast-response probes for the detection of transient (millisecond) potential changes. Slow-response dyes function by entering depolarized cells and binding to proteins or membranes. Increased depolarization results in additional dye influx and an increase in fluorescence, while hyperpolarization is indicated by a decrease in fluorescence. Fast-response probes are commonly used to image electrical activity from intact heart tissues or measure membrane potential changes in response to pharmacological stimuli. Slow-responding probes are often used to explore mitochondrial function and cell viability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What type of membrane potential indicators do you offer and how should I choose one for my experiment?

A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regulation/ion-indicators/membrane-potential-indicators.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (120)

Citations & References
Abstract
Use of a membrane potential-sensitive probe to assess biological expression of the cystic fibrosis transmembrane conductance regulator.
Authors:Renier M, Tamanini A, Nicolis E, Rolfini R, Imler JL, Pavirani A, Cabrini G
Journal:Hum Gene Ther
PubMed ID:8590731
Cystic fibrosis is caused by defects in a chloride-transporting protein termed cystic fibrosis transmembrane conductance regulator (CFTR). This study presents an innovative procedure to evaluate expression of functional CFTR. The technique uses the potential-sensitive probe bis-(1,3-diethylthiobarbituric acid) trimethine oxonol or DiSBAC2(3), by single-cell fluorescence imaging. The DiSBAC2(3) method was first ... More
Expression of the voltage-gated sodium channel NaV1.5 in the macrophage late endosome regulates endosomal acidification.
Authors:Carrithers MD, Dib-Hajj S, Carrithers LM, Tokmoulina G, Pypaert M, Jonas EA, Waxman SG,
Journal:J Immunol
PubMed ID:17548620
'Voltage-gated sodium channels expressed on the plasma membrane activate rapidly in response to changes in membrane potential in cells with excitable membranes such as muscle and neurons. Macrophages also require rapid signaling mechanisms as the first line of defense against invasion by microorganisms. In this study, our goal was to ... More
Heat shock- and ethanol-induced ionic changes in C6 rat glioma cells determined by NMR and fluorescence spectroscopy.
Authors:Skrandies S, Bremer B, Pilatus U, Mayer A, Neuhaus-Steinmetz U, Rensing L
Journal:Brain Res
PubMed ID:9037501
'The effects of two different stressors, heat shock (HS; 44 degrees C, 20 min) and ethanol (1.2 M, 60 min), on ion content and membrane potential were investigated in C6 rat glioma cells. Both treatments were previously shown to induce the HS response [26]. Intracellular pH (pH(i)), sodium ion concentration ... More
Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages.
Authors:Haslberger A, Romanin C, Koerber R
Journal:Mol Biol Cell
PubMed ID:1498365
'Lipopolysaccharide (LPS)-mediated synthesis of macrophage gene products such as tumor necrosis factor (TNF) is controlled by different signaling pathways. We investigated intracellular free Ca2+ (Ca2+ic) and the membrane potential as early cellular responses to LPS and their role in the synthesis and release of TNF. In peritoneal macrophages and in ... More
Selective modulation by cGMP of the K+ channel activated by speract.
Authors:Cook SP, Babcock DF
Journal:J Biol Chem
PubMed ID:7693667
'The egg peptide speract stimulates sperm guanylyl cyclase and presumably enhances fertilization, but the roles of cGMP in sperm responses are yet undetermined. Here we show that speract-induced accumulation of cGMP or cAMP is selectively enhanced by the phosphodiesterase inhibitors, 3-isobutyl-1-methylxanthine (IBMX) or papaverine, respectively. These inhibitors provided the unusual ... More
View all DiSBAC2(3) (Bis-(1,3-Diethylthiobarbituric Acid)Trimethine Oxonol) citations