IL-4 Human ELISA Kit, High Sensitivity

Catalog number:  BMS225HS

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

This High Sensitivity ELISAs enables the analysis of Interleukin- 4 in low concentrations from serum, plasma, or cell culture samples.
Our High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4.
IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

Conjugate
Biotin

Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Sample Volume
50 µL

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Assay Range: 0.25-16.0 pg/mL
Assay Sensitivity: 0.1 pg/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: eBioscience
Gene Aliases: BCGF-1, BCGF1, BSF-1, BSF1, IL-4
Gene ID: 3565
Gene Name: interleukin 4
Gene Symbol: IL4
Immunoassay Kit Format: Coated ELISA Kit
Immunoassay Kit Grade: High Sensitivity
Interassay CV: 11.0%
Intraassay CV: 8.8%
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Interleukin-4
Sample Type (Specific): Cell Culture Supernatants, Plasma (EDTA, Citrate, Heparin), Serum
Sample Volume: 50 µL
Target (Kit Named): IL-4
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P05112

Contents & storage

Aluminium pouch(es) with a Microplate coated with monoclonal antibody to human IL-4
Biotin-Conjugate anti-human IL-4 polyclonal antibody
Streptavidin-HRP
Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I*
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution
Stop Solution (1M Phosphoric acid)
Adhesive Films
* reagent contains ethyl alcohol

Documents

Manuals & protocols