VEGF Human ELISA Kit

Catalog number: BMS277-2

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The human VEGF-A ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human VEGF-A.

One of the key molecules for angiogenesis and for the survival of the endothelium is vascular endothelial growth factor (VEGF-A). It is a specific endothelial cell mitogen and a strong vascular permeability factor (VPF).
VEGF-A is a heparin-binding glycoprotein, secreted as a homodimer of 45 kDa by many different cell types. VEGF-A also causes vasodilation through the nitiric oxide synthase pathway in endothelial cells and can activate migration in monocytes. Many different splice variants of VEGF-A have been described, but VEGF165 is the most predominant protein and anchors with its heparin binding domain to extracellular matrix and to heparin sulfate.

Examples where VEGF-A plays an important role are psoriasis andrheumatoid arthritis, as well as the ovarian hyperstimulation syndrome. Diabetic retinopathy is associated with high intraocular levels of VEGF-A, and inhibition of VEGF-A function may result in infertility by blockage of corpus luteum function. Direct demonstration of the importance of VEGF-A in tumor growth has been achieved using dominant negative VEGF receptors to block in vivo proliferation, as well as blocking antibodies to VEGF or to one of the VEGF receptors. VEGF-A transcription is highly activated by hypoxia and by oncogenes like H-ras and several transmembrane tyrosine kinases, such as epidermal growth factor receptor and ErbB2. Together these pathways account for a marked upregulation of VEGF-A in tumors compared to normal tissues and are often of prognostic importance and relevance. Targeting the VEGF signalling may be of major therapeutic importance for many diseases and serves as a basis for the design of future (anti)-angiogenic treatments.

Conjugate
Biotin

Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Sample Volume
50 µL

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: 15.6-1,000 pg/mL
Assay Sensitivity: 7.9 pg/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: Platinum, eBioscience
Gene Aliases: MVCD1, VEGF, VPF
Gene ID: 7422
Gene Name: vascular endothelial growth factor A
Gene Symbol: VEGFA
Immunoassay Kit Format: Coated ELISA Kit
Interassay CV: 4.3%
Intraassay CV: 6.2%
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Aliases: VEGF-A, Vascular permeability factor, VPF
Protein Name: vascular endothelial growth factor A
Sample Type (Specific): Cell Culture Supernatants, Plasma (EDTA, Citrate, Heparin), Serum
Sample Volume: 50 µL
Target (Kit Named): VEGF
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P15692

Documents

Manuals & protocols