Informational:Informational:Detect and image cellular apoptosis »
Invitrogen™

Click-iT™ TUNEL Alexa Fluor Imaging Assays for Microscopy & HCS

Green features
Catalog number: C10247
Invitrogen™

Click-iT™ TUNEL Alexa Fluor Imaging Assays for Microscopy & HCS

Green features
Catalog number: C10247
Screen apoptotic cells easily and efficiently in just two hours with the Click-iT TUNEL Alexa Fluor 488, 594, and 647 imaging assays. Intended for use with microscopy and high-content screening (HCS), these TUNEL assay kits use a copper-catalyzed reaction to detect incorporated alkyne-modified dUTPs on terminal DNA ends of fragmented DNA. Compared with assays using other modified nucleotides, these apoptosis detection kits are fast (complete within two hours) and can detect a higher percentage of apoptotic cells under identical conditions. Click-iT TUNEL assays also allow multiplexing with surface and intracellular biomarker detection methods, including those that measure fluorescent signal from GFP or RFP.
 
Catalog Number
C10247
Unit Size
1 kit
Color
Far-Red
Label or Dye
Alexa Fluor™ 647, Hoechst 33342
No. of Reactions
1 X 96 tests or 50 coverslips
Price (USD)
1,058.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionClick-iT TUNEL Alexa Fluor™ 647 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission650/665
No. of Reactions1 X 96 tests or 50 coverslips
ColorFar-Red
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 647, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protected from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Red
Label or Dye
Alexa Fluor™ 594, Hoechst 33342
No. of Reactions
1 X 96 tests or 50 coverslips
Price (USD)
1,064.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionClick-iT TUNEL Alexa Fluor™ 594 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission590/617
No. of Reactions1 X 96 tests or 50 coverslips
ColorRed
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 594, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Color
Green
Label or Dye
Alexa Fluor™ 488, Hoechst 33342
No. of Reactions
1 X 96 tests or 50 coverslips
Price (USD)
1,084.00
Your Price:
Availability
***
Full specifications
Quantity
DescriptionClick-iT TUNEL Alexa Fluor™ 488 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission490/525
No. of Reactions1 X 96 tests or 50 coverslips
ColorGreen
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 488, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protected from light.
Unit Size1 kit
Showing 3 of 3
Catalog NumberSpecificationsUnit SizeColorLabel or DyeNo. of ReactionsPrice (USD)AvailabilityQuantity
C10247Full specifications
1 kitFar-RedAlexa Fluor™ 647, Hoechst 333421 X 96 tests or 50 coverslips
1,058.00
Your Price:
***
DescriptionClick-iT TUNEL Alexa Fluor™ 647 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission650/665
No. of Reactions1 X 96 tests or 50 coverslips
ColorFar-Red
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 647, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protected from light.
Unit Size1 kit
C10246Full specifications
1 kitRedAlexa Fluor™ 594, Hoechst 333421 X 96 tests or 50 coverslips
1,064.00
Your Price:
***
DescriptionClick-iT TUNEL Alexa Fluor™ 594 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission590/617
No. of Reactions1 X 96 tests or 50 coverslips
ColorRed
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 594, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protect from light.
Unit Size1 kit
C10245Full specifications
1 kitGreenAlexa Fluor™ 488, Hoechst 333421 X 96 tests or 50 coverslips
1,084.00
Your Price:
***
DescriptionClick-iT TUNEL Alexa Fluor™ 488 Imaging Assay
Format96-well Plate
Product TypeImaging Assay
Excitation/Emission490/525
No. of Reactions1 X 96 tests or 50 coverslips
ColorGreen
Shipping ConditionDry Ice
Product LineClick-iT
Detection MethodFluorescent
For Use With (Equipment)Fluorescence Microscope, High Content Instrument
Label or DyeAlexa Fluor™ 488, Hoechst 33342
Label TypeAlexa Fluor™ Dyes, Classic Dyes
Storage RequirementsStore at ≤-20°C and protected from light.
Unit Size1 kit
Showing 3 of 3
Apoptosis is marked by defined cellular processes, including cell rounding, blebbing, and DNA fragmentation. The TUNEL assay is the most widely used analytical method to detect fragmented DNA in apoptotic cells in tissue samples, beginning with the incorporation of modified dUTPs at the 3’-OH ends of fragmented DNA. The dUTPs often include a fluorophore molecule. Due to the size of the fluorophore, modified dUTPs can display lower than expected incorporation rates, negatively affecting the sensitivity of the TUNEL assay. Quite often, fluorophores used in currently available TUNEL assay kits exhibit photobleaching and fluorescent spectral overlap issues, both of which reduce the sensitivity of and ability to multiplex these assays.

The Click-iT Plus TUNEL assay was developed to address these issues and is based on a copper (I) catalyzed (i.e., click) reaction between an azide and alkyne. The small size of the Alexa Fluor azide, which has a molecular weight of about 1 kDa, enables easier incorporation of the Alexa Fluor 488, 594, or 647 dye into complex samples, as compared to larger (MW ˜100,000 Da) antibodies. This permits mild fixation or permeabilization and faster assay turnaround time (about two hours) and enables the detection of a higher percentage of apoptotic cells under identical conditions. Because of its gentle reaction conditions, the Click-iT Plus TUNEL assay can also be multiplexed with fluorescent proteins or dyes.

The Click-iT TUNEL Alexa Fluor imaging assays contain all components needed to accurately and reliably detect apoptosis in adherent cells grown on coverslips or 96-well microplates, and they also include DNase I to generate strand breaks for the positive control.

Figures