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Invitrogen™

Click-IT™ Palmitic Acid, Azide (15-Azidopentadecanoic Acid) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  C10265

Related applications:

Labeling Chemistry

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Description

Identify and characterize palmitylated proteins with Click-iT® palmitic acid, azide using the powerful click chemistry, a simple and robust two-step labeling and detection technique. In step one, the azide-containing biomolecule is fed to cells or animals and actively incorporated into proteins. Unlike other labels such as biotin or a fluorescent dye, the azide-tag is small enough that the tagged molecule is an acceptable substrate for the enzymes that incorporate this building block into proteins. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the modified protein is detected with the corresponding alkyne-containing dye or hapten using either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS) together with other biomarkers of interest for content and context rich results. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solid
Labeling Method: Metabolic Labeling
Labeling Target: Proteins (Nascent), Proteins (General), Proteins (Cell Surface)
Shipping Condition: Room Temperature
Product Line: Click-iT™, Molecular Probes®
Label or Dye: Azide
Green Features: Less hazardous

Contents & storage

Store at ≤-20°C, desiccated and protected from light.