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Invitrogen™

Image-iT™ Lipid Peroxidation Kit, for live cell analysis

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Description

The Image-iT® Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY® 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ~590 nm to ~510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells.

Additional Information:

• The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Green
Detection Method: Fluorescent
Form: Frozen
For Use With (Equipment): Floid® Cell Imaging System, Fluorescence Microscope, Flow Cytometer, High Content Instrument
Indicator: Lipid Peroxidation Sensor
Solubility: DMSO (Dimethylsulfoxide)
Sub Cellular Localization: Cytoplasm
Shipping Condition: Wet Ice
Product Line: Image-iT®

Contents & storage

Store component A store at ≤-20°C

Store component B at room temperature