Click-iT® Plus Alexa Fluor® picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).
• Flexible—optimize your own Click–iT® reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples • Bright and sensitive—superior fluorescent Alexa Flour® dyes combined with the efficiency of picolyl azide • Convenient—contains all the buffers and reagents needed to create the optimal click reaction
Each Click-iT® Plus Picolyl Azide Toolkit includes 100 µg of Alexa Fluor® dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT® Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.
For Research Use Only. Not for use in diagnostic procedures.
Alexa Fluor Dyes
Label or Dye:
Alexa Fluor™ 488
Contents & storage
• 1 x 100 µg Alexa Fluor™ dye-picolyl azide • 1 x 4 mL Click-iT™ reaction buffer • 1 x 1.5 mL copper (II) sulfate • 1 x 500 µL copper protectant • 1 Click-iT™ buffer additive
Each kit contains enough material for approximately 25 to 100 reactions, depending on application.