Click-iT™ EdU Colorimetric IHC Detection Kit

Click-iT™ EdU Colorimetric IHC Detection Kit - FAQs

View additional product information for Click-iT™ EdU Colorimetric IHC Detection Kit - FAQs (C10644)

2 product FAQs found

I have the Click-iT EdU Colorimetric IHC Detection Kit, but have decided that I want to use a fluorescent label but I don't want to buy another kit. What can I do?

You need to only substitute the peroxidase-conjugated streptavidin with a dye-conjugated streptavidin and not use the DAB. You would have to optimize the final concentration of the dye-conjugated streptavidin to use.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can other peroxidase substrates be used with the Click-iT EdU Colorimetric IHC Detection Kit instead of the DAB?

Yes, but we have not optimized the amount of substrate to use. The labeling may be punctate and therefore an intense dark label such as DAB is recommended.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.