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CellEvent™ Senescence Green Flow Cytometry Assay Kit, 50 Assays - FAQs

CellEvent™ Senescence Green Flow Cytometry Assay Kit, 50 Assays - FAQs

View additional product information for CellEvent™ Senescence Green Flow Cytometry Assay Kit - FAQs (C10841, C10840)

4 product FAQs found

I am multiplexing CellEvent Senescence Green Flow Cytometry Assay Kit (Cat. No. C10840, C10841) with another method. Can I skip the PFA fixation step?

We do not recommend skipping the fixation step when using CellEvent Senescence Green Flow Cytometry Assay Kit, as it is a necessary step. The CellEvent Senescence Green probe only works on fixed cells.

Can the CellEvent Senescence Green Probe be multiplexed using antibodies for intracellular labeling?

Yes. After incubating the cells with CellEvent Senescence Green Probe, permeabilize the cells, wash, and proceed with detection.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use my own buffer with the CellEvent Senescence Green Probe?

We do not recommend using your own buffer with the CellEvent Senescence Green Probe. The assay is pH sensitive and we recommend that you use the buffer provided in the kit be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the CellEvent Senescence Green Probe on live cells?

No. It only works on fixed cells; b-galactosidase is still active immediately following fixation with paraformaldehyde.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.