SiteClick™ sDIBO Alkyne Kits for Antibody Labeling
The SiteClick™ sDIBO Alkyne Kits are optimized to be used in conjunction with our SiteClick™ Antibody Azido Modification Kits
SiteClick™ sDIBO Alkyne Kits for Antibody Labeling
Invitrogen™

SiteClick™ sDIBO Alkyne Kits for Antibody Labeling

Easily attach Alexa Fluor™ pHrodo™ and Biotin sDIBO Alkyne tags to azido-modified antibodies using SiteClick™ chemistry.
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Catalog NumberLabel or DyeLabeling Scale
C20034pHrodo Red100-250 μg
C20027Alexa Fluor 488100-250 μg
C20029Alexa Fluor 647100-250 μg
C20031Primary Amine100-250 μg
S10902Biotin1 mg
S10903pHrodo Red1 mg
S10904Alexa Fluor 4881 mg
S10906Alexa Fluor 6471 mg
S10907Biotin5 mg
S10908pHrodo Red5 mg
S10909Alexa Fluor 4885 mg
S10911Alexa Fluor 6475 mg
S10914pHrodo Deep Red100-250 μg
S10915pHrodo Deep Red1 mg
C20028Alexa Fluor 555100-250 μg
C20030Biotin100-250 μg
16 Options
Catalog number C20034
Price (USD)/ 1 kit
327.65
Online exclusive
334.00 
Save 6.35 (2%)
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Label or Dye:
pHrodo Red
Labeling Scale:
100-250 μg
Price (USD)/ 1 kit
327.65
Online exclusive
334.00 
Save 6.35 (2%)
Add to cart
SiteClick™ sDIBO Alkyne Kits for Antibody Labeling
Catalog numberC20034
Price (USD)/ 1 kit
327.65
Online exclusive
334.00 
Save 6.35 (2%)
-
Add to cart
Generate site-specific labeled antibody conjugates with SiteClick sDIBO Alkynes for Antibody Labeling. Our SiteClick sDIBO Antibody Labeling kits are optimized to be used in conjunction with the SiteClick Azido Modification kits (S20026, S10900, or S10901 sold separately) and contain sufficient reagents to perform a conjugation reaction of a SiteClick sDIBO Alkyne label using 100 μg, 1 mg, or 5 mg of azido-modified IgG antibody. The SiteClick sDIBO Alkynes for Antibody Labeling employs copper-free click chemistry to covalently link a dibenzocyclooctyne (sDIBO) labeled Alexa Fluor dye, pHrodo dye, or biotin to an azido modified antibody. The label, when attached to the antibody, is located far away from its antigen binding domain.

Create your own covalently labeled antibody conjugates using the SiteClick sDIBO Alkyne labels, which are intended for use with azido-modified antibodies. SiteClick sDIBO Alkyne Antibody Labeling Kits include the sDIBO Alkyne and the antibody concentrator, enabling copper-free covalent attachment of Alexa Fluor and pHrodo dyes, biotin, or molecules of your choice. When used with the SiteClick Antibody Azido Modification Kit (available separately as Cat. No. S20026, S10900, S10901), labeling occurs on the heavy chain N-linked glycans, far away from the antigen-binding domain, enabling excellent reproducibility between separate labeling reactions and multiple antibody species and subtypes. The SiteClick sDIBO alkyne kits for antibody labeling are optimized for conjugation reactions, covalently linking 100 μg, 1 mg, or 5 mg of azide-modified IgG antibody.

The SiteClick Antibody Azido Modification Kit (Cat. No. S20026, S10900, S10901) is recommended for the attachment of an azide moiety to the heavy chains of an unlabeled IgG antibody. Other engineered antibodies containing azido moieties are also suitable.

When used in conjunction with the SiteClick Antibody Azido Modification Kit, the SiteClick sDIBO alkyne kits for antibody labeling give you the option of trying different fluorescent labels for your antibody, attaching other molecules via streptavidin, or attaching your own molecule via amine-reactive moieties, depending on your assay. Our sDIBO alkynes are improved versions of DIBO cyclooctynes, yielding conjugates that are less 'sticky' and emit lower signal background in biological samples.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Kit ContentsContains 1 vial of SiteClick pHrodo iFL Red sDIBO Alkyne for Antibody Labeling, 1 vial of DMSO, and 1 antibody concentrator
Filter TypeRFP, Texas Red
Chemical ReactivityAzide
Flow Cytometer Laser LinesBlue, Yellow
Labeling MethodClick Chemistry
Labeling Scale100-250 μg
Shipping ConditionWet Ice
Reactive MoietysDIBO Alkyne
Label or DyepHrodo Red
ColorRed
Product LinepHrodo™, SiteClick™
Labeling TargetAzido modified antibodies
Excitation/Emission560/585 nm
Unit Size1 kit
Contents & Storage
Store in refrigerator (2°C to 8°C) and protect from light.