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Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA

Catalog number: C3008MP

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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The Calcium Calibration Buffer Kits are designed to help determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a fluorometer or quantitative imaging system. The Kd of an indicator can be calculated from a plot generated by scanning the excitation or emission of the indicator in the presence of eleven different Ca2+ concentrations. Calcium Calibration Kit #1 contains 50 mL of 10 mM K2EGTA and 50 mL of 10 mM CaEGTA. Both solutions contain 100 mM KCl and 30 mM MOPS, pH 7.2 and are prepared in deionized water. These stock solutions can be blended to make buffers having free Ca2+ ranging from 0 µM to 39 µM.
For Research Use Only. Not for use in diagnostic procedures.


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Indicator Type: Ion Calibration Reagent
For Use With (Equipment): Fluorescence Microscope, Fluorometer
Product Size: 1 kit
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols

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