Invitrogen™

Cascade Blue™ hydrazide, Trilithium Salt

Catalog number:  C3239

Related applications:

Cell Analysis

| Cell Analysis

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Description

The cell-impermeant, fixable, polar tracer Cascade Blue® hydrazide can be used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The primary aliphatic amine of Cascade Blue® hydrazide can also be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
Reactive Moiety: Amine, Hydrazide
Shipping Condition: Room Temperature
Product Line: CASCADE BLUE®
Label or Dye: Cascade Blue®
Label Type: Classic Dyes

Contents & storage

Store at room temperature and protect from light.