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Click-IT™ O-GlcNAc Enzymatic Labeling System This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

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The Click-iT® O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT® Glycoprotein Detection kit for gels (TAMRA or Dapoxyl® alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT® glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Biotin-Based, Fluorescent
Green Features: Less hazardous
Label or Dye: Alexa Fluor™ 488, Alexa Fluor™ 555, Alexa Fluor™ 594, Alexa Fluor™ 647, Biotin, Oregon Green® 488, TMR (Tetramethylrhodamine)
Labeling Target: Proteins (Glycoproteins)
Product Line: Click-iT™
Product Size: 1 kit
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols