Invitrogen™

CyQUANT™ Cell Proliferation Assay, for cells in culture

Catalog number: C7026
Invitrogen™

CyQUANT™ Cell Proliferation Assay, for cells in culture

Catalog number: C7026
Catalog Number
C7026
Unit Size
1,000 assays
Price (USD)
Price: 407.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C70261,000 assays
Price: 407.00
Your Price:
-
Product Overview
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The CyQUANT® Cell Proliferation Assay is a highly sensitive fluorescence-based method for quantifying cells and assessing cell proliferation and cytotoxicity. For cell proliferation assays that are more compatible with high throughput screening, see our CyQUANT® NF Cell Proliferation Assay or our CyQUANT® Direct Assay.

With the CyQUANT® Cell Proliferation Assay, you can:

• Measure DNA content to directly quantify 50 to 50,000 cells per well without relying on metabolic activity
• Quickly compare multiple samples in a simple plate-based assay format
• Freeze and store time course samples for later analysis

Measure DNA content directly
The main component of the CyQUANT® Cell Proliferation Assay is CyQUANT® GR, a proprietary dye that exhibits strong fluorescence enhancement when bound to nucleic acids. With this dye, you can measure a sample's DNA content and compare it to standards, directly quantifying the entire cell population within a broad linear detection range. This method offers improved accuracy over metabolically based cell proliferation or cytotoxicity assays that can be influenced by cell changes that are unrelated to cell number.

Quickly compare multiple samples
The CyQUANT® Cell Proliferation Assay requires minimal hands-on time. Simply remove the culture media, freeze and lyse cells, then read fluorescence on a standard microplate reader with a fluorescein filter.

Store samples for later analysis
Because the cells are frozen, samples can stored for up to 4 weeks and run in batches, allowing the direct comparison of samples taken at different time points.

Kit components
The kit includes CyQUANT® GR dye and cell lysis buffer sufficient to perform 1,000 assays in volumes suitable for fluorescence detection in microplates. The kit also contains bacteriophage λ DNA to be used as a reference standard for assay calibration.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability
Cell-impermeant
Color
Green
Description
CyQUANT™ Cell Proliferation Assay, for cells in culture
Detection Method
Fluorescence
For Use With (Application)
Proliferation Assay
For Use With (Equipment)
Microplate Reader
Product Type
Cell Proliferation Assay
Dye Type
Other Label(s) or Dye(s)
Emission
520 nm
Excitation Wavelength Range
480 nm
Format
96-well plate
Product Line
CyQUANT™
Quantity
1000 Assays
Shipping Condition
Room Temperature

Contents & Storage

Contains CyQUANT™ GR dye, cell lysis buffer, and bacteriophage λ DNA.