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OriGene
FADD Monoclonal Antibody (OTI1C11), TrueMAB™
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FIGURE: 1 / 3
FADD Antibody (CF501434) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FADD (RC201805, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FADD. Positive lysates LY401265 (100 µg) and LC401265 (20 µg) can be purchased separately from OriGene.
Product Details
CF501434
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1C11
Immunogen
Full length human recombinant protein of human FADD produced in HEK293T cell.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FADD; Fas (TNFRSF6)-associated via death domain; FAS-associated death domain protein; FAS-associating death domain-containing protein; Fas-associating protein with death domain; GIG3; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; mediator of receptor-induced toxicity; MGC8528; unnamed protein product
Gene Aliases: FADD; GIG3; IMD90; MORT1
UniProt ID: (Human) Q13158
Entrez Gene ID: (Human) 8772
protease binding
death receptor binding
tumor necrosis factor receptor binding
protein binding
protein binding, bridging
tumor necrosis factor receptor superfamily binding
receptor serine/threonine kinase binding
signaling adaptor activity
death effector domain binding
identical protein binding
macromolecular complex binding
caspase binding
positive regulation of cytokine production
kidney development
positive regulation of T cell mediated cytotoxicity
immune system process
positive regulation of adaptive immune response
apoptotic process
signal transduction
extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of interferon-gamma production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
T cell differentiation in thymus
TRAIL-activated apoptotic signaling pathway
positive regulation of activated T cell proliferation
response to cocaine
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of innate immune response
positive regulation of macrophage differentiation
positive regulation of proteolysis
positive regulation of transcription from RNA polymerase II promoter
behavioral response to cocaine
lymph node development
spleen development
thymus development
positive regulation of immune response
defense response to virus
positive regulation of type I interferon-mediated signaling pathway
regulation of necroptotic process
negative regulation of necroptotic process
negative regulation of activation-induced cell death of T cells
cellular response to mechanical stimulus
death-inducing signaling complex assembly
motor neuron apoptotic process
apoptotic signaling pathway
extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
necroptotic signaling pathway
defense response to other organism
positive regulation of execution phase of apoptosis
positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation
positive regulation of extrinsic apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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