Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Label or Dye |
---|---|
D20655 | Biotin (DSB-X) |
A10170 | Alexa Fluor 350 |
A10235 | Alexa Fluor 488 |
A10236 | Alexa Fluor 532 |
A10237 | Alexa Fluor 546 |
A20174 | Alexa Fluor 555 |
A10238 | Alexa Fluor 568 |
A10239 | Alexa Fluor 594 |
A20170 | Alexa Fluor 633 |
A20173 | Alexa Fluor 647 |
A20171 | Alexa Fluor 660 |
A20172 also known as A-20172 | Alexa Fluor 680 |
F10240 | FITC (Fluorescein) |
O10241 also known as O-10241 | Oregon Green 488 |
P30012 | Pacific Blue |
Ready to use in just 90 minutes, our protein labeling kits include an easy-to-use, pre-packed spin column for rapid dye removal and typical recovery greater than 85%. Each kit contains enough reagent for 3–5 protein conjugation reactions. Our kits provide better results due to lower background fluorescence, less nonspecific binding, and easier workflows for protein conjugation with 16 different fluorophores.
Each protein labeling kit contains everything you need to perform 3-5 separate labeling reactions and purify the resulting conjugates. The reactive dye has either a succinimidyl ester (SE) or a tetrafluorophenyl (TFP) ester moiety that reacts efficiently with primary amines of proteins to form stable dye–protein conjugates. Each of the vials of reactive dye provided in the kit is sufficient for labeling 1 mg of a variety of purified proteins, including growth factors, cytokines, nanobodies, enzymes, cell-adhesion molecules, and antibodies.
Direct labeling with fluorophores allows multiple primary antibodies of the same isotype (derived from the same species) to be used in the same experiment. Stabilizing proteins such as BSA should be removed from the sample before labeling.
The different protein labeling kits
For labeling smaller amounts of antibodies (∼100 μg), we recommend our antibody labeling kits. Please review the protein labeling kit user manual for more in depth information, protocols, molecular weights, and degree of labeling for each dye.