What is the difference between BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) (Cat. No. D3922) and BODIPY 493/503 NHS Ester (Succinimidyl Ester) (Cat. No. D2191)?
BODIPY 493/503 NHS Ester (Succinimidyl Ester) (Cat. No. D2191) is the amine‐reactive derivative of BODIPY 493/503 (4,4‐Difluoro‐1,3,5,7,8‐Pentamethyl‐4‐Bora‐3a,4a‐Diaza‐s‐Indacene) (Cat. No. D3922). The reactive group allows for D2191 to be used as a labeling reagent to label proteins or other molecules to covalently attach the dye to accessible primary amines. If using this as a live cell reagent, the reactive group may allow for better retention as it will covalently attach to any primary amines on any cellular material it may come into contact with.
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Find additional tips, troubleshooting help, and resources within our Cell Imaging Support Center.