AuthorsHara T, Hirasawa A, Sun Q, Koshimizu TA, Itsubo C, Sadakane K, Awaji T, Tsujimoto G,
JournalMol Pharmacol
PubMed ID18927207
'GPR40 is a G protein-coupled receptor (GPCR) whose endogenous ligands have recently been identified as medium- and long-chain free fatty acids (FFAs), and it is thought to play an important role in insulin release. Despite recent research efforts, much still remains unclear in our understanding of its pharmacology, mainly because ... More
'The spectroscopic properties of a new series of fatty acid analogs in which a dipyrrometheneboron difluoride fluorophore forms a segment of the acyl methylene chain are presented and their characteristics as fluorescent membrane probes are examined. When incorporated as a low mole fraction component in model phospholipid membranes, the probes ... More
Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production.
AuthorsBrennan L, Blanco Fernández A, Mostaert AS, Owende P,
JournalJ Microbiol Methods
PubMed ID22521923
'This paper describes a microalgal cell lipid fluorescence enhancement method using BODIPY(505/515), which can be used to screen for lipids in wild-type microalgae and to monitor lipid content within microalgae production processes to determine optimal harvesting time. The study was based on four microalgae species (Dunaliella teteriolecta, Tetraselmis suecica, Nannochloropsis ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
Increased lipid accumulation in the Chlamydomonas reinhardtii sta7-10 starchless isoamylase mutant and increased carbohydrate synthesis in complemented strains.
AuthorsWork VH, Radakovits R, Jinkerson RE, Meuser JE, Elliott LG, Vinyard DJ, Laurens LM, Dismukes GC, Posewitz MC,
JournalEukaryot Cell
PubMed ID20562225
'The accumulation of bioenergy carriers was assessed in two starchless mutants of Chlamydomonas reinhardtii (the sta6 [ADP-glucose pyrophosphorylase] and sta7-10 [isoamylase] mutants), a control strain (CC124), and two complemented strains of the sta7-10 mutant. The results indicate that the genetic blockage of starch synthesis in the sta6 and sta7-10 mutants ... More
Analyzing morphogenetic cell behaviors in vitally stained zebrafish embryos.
We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content. ... More
The life of lipid droplets.
AuthorsWalther TC, Farese RV,
JournalBiochim Biophys Acta
PubMed ID19041421
Lipid droplets are the least characterized of cellular organelles. Long considered simple lipid storage depots, these dynamic and remarkable organelles have recently been implicated in many biological processes, and we are only now beginning to gain insights into their fascinating lives in cells. Here we examine what we know of ... More
Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae.
AuthorsPereira H, Barreira L, Mozes A, Florindo C, Polo C, Duarte CV, Custódio L, Varela J,
JournalBiotechnol Biofuels
PubMed ID22192119
We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-rich microalgae from an environmental sample. Our results show that direct sorting onto solid medium upon FACS can save about 3 weeks during the scale-up process as compared with the ... More
Comparative photostability studies of BODIPY and fluorescein dyes by using fluorescence correlation spectroscopy.
AuthorsHinkeldey B, Schmitt A, Jung G,
JournalChemphyschem
PubMed ID18816535
In single-molecule applications, the photostability of fluorescent molecules is a key parameter. We apply fluorescence correlation spectroscopy to compare the photostability of four fluorescein and four borondipyrromethene (BODIPY) dyes of similar structure but different triplet yields. The latter class of dyes are more stable. In the kinetic analysis the, diffusion ... More
Pathfinding of olfactory neuron axons to stereotyped glomerular targets revealed by dynamic imaging in living zebrafish embryos.
AuthorsDynes JL, Ngai J
JournalNeuron
PubMed ID9655497
In the vertebrate olfactory system, sensory neurons with common odorant specificities project to specific glomeruli in the olfactory bulb. How do olfactory sensory neurons find their glomerular targets? To address this question, we have visualized the genesis of the peripheral olfactory system in living zebrafish embryos. Dye labelings reveal that ... More
Synthesis, chiroptical properties, and solid-state structure determination of two new chiral dipyrrin difluoroboryl chelates.
AuthorsGossauer A, Nydegger F, Kiss T, Sleziak R, Stoeckli-Evans H
JournalJ Am Chem Soc
PubMed ID14871109
Two new types of optically active BODIPY fluorophores bearing chiral phenyl substituents either at the meso-position or at both external alpha-positions have been synthesized. Their chiroptical properties are strongly dependent both on the position of the chiral group and on the protonation of the chromophore. The solid-state structures of one ... More
A comparison of lipid storage in Phaeodactylum tricornutum and Tetraselmis suecica using laser scanning confocal microscopy.
AuthorsWong DM, Franz AK,
Journal
PubMed ID23933493
Microalgae contain lipid bodies (LBs) composed of triacylglycerols, which can be converted to biodiesel. Here we demonstrate a method to study the accumulation patterns of LBs in different microalgae strains and culture conditions utilizing laser scanning confocal microscopy (LSCM) with BODIPY 505/515 (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, in parallel with Nile Red (9-diethylamino-5H-benzo-a-phenoxazine-5-one) ... More
In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules.
AuthorsLee DH, Bae CY, Han JI, Park JK,
Journal
PubMed ID24007509
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source and carbon dioxide as an only carbon source, have been considered as a feedstock of choice for the production of biofuels such as biodiesel. To explore the economic feasibility of such application, however, many technical hurdles ... More
Confocal microscopic analysis of morphogenetic movements.
AuthorsCooper MS, D'Amico LA, Henry CA
JournalMethods Cell Biol
PubMed ID9891361
Confocal microscopy is an excellent means of imaging cellular dynamics within living zebrafish embryos because it provides a means of optically sectioning tissues that have been labeled with specific fluorescent probe molecules. In order to study genetically encoded patterns of cell behavior that are involved in the formation of germ ... More
Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore-rational design of potentially useful bioimaging fluorescence probe.
AuthorsGabe Y, Urano Y, Kikuchi K, Kojima H, Nagano T
JournalJ Am Chem Soc
PubMed ID15012166
Boron dipyrromethene (BODIPY) is known to have a high quantum yield (phi) of fluorescence in aqueous solution but has not been utilized much for biological applications, compared to fluorescein. We developed 8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (DAMBO-P(H)), based on the BODIPY chromophore, as a highly sensitive fluorescence probe for nitric oxide (NO). DAMBO-P(H) had ... More
The Incubascope: a simple, compact and large field of view microscope for long-term imaging inside an incubator.
Authors
JournalR Soc Open Sci
PubMed ID35154792
SREBP1-Induced Glutamine Synthetase Triggers a Feedforward Loop to Upregulate SREBP1 through Sp1 O-GlcNAcylation and Augments Lipid Droplet Formation in Cancer Cells.
Authors
JournalInt J Mol Sci
PubMed ID34575972
Seemingly trivial secondary factors may determine microbial competition: a cautionary tale on the impact of iron supplementation through corrosion.
Authors
JournalFEMS Microbiol Ecol
PubMed ID33428722
Proteomics Analysis of Lipid Droplets from the Oleaginous Alga Chromochloris zofingiensis Reveals Novel Proteins for Lipid Metabolism.
Authors
JournalGenomics Proteomics Bioinformatics
PubMed ID31494267
Lens apoptosis in the Astyanax blind cavefish is not triggered by its small size or defects in morphogenesis.
Authors
JournalPLoS One
PubMed ID28235048
High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica.
Authors
JournalMetab Eng
PubMed ID33971293
Fatty acid metabolism complements glycolysis in the selective regulatory T cell expansion during tumor growth.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID29941600
All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide.
Authors
JournalSci Rep
PubMed ID28186188
A Gelatin Microdroplet Platform for High-Throughput Sorting of Hyperproducing Single-Cell-Derived Microalgal Clones.