Zitierungen und Referenzen (1016)

Invitrogen™

H2DCFDA (H2-DCF, DCF)

Das zelldurchlässige 2',7'-Dichlordihydrofluorescein-diacetat (H2DCFDA) (auch bekannt als Dichlorfluores cin -diacetat) ist eine chemisch reduzierte Form des Fluorescein, die als IndikatorWeitere Informationen

Katalognummer	Menge
D399	100 mg

Katalognummer D399

Preis (EUR)

269,65

Exklusiv online

293,00

Ersparnis 23,35 (8%)

Menge:

100 mg

Preis (EUR)

269,65

Exklusiv online

293,00

Ersparnis 23,35 (8%)

Das zelldurchlässige 2',7'-Dichlordihydrofluorescein-diacetat (H₂DCFDA) (auch bekannt als Dichlorfluores cin -diacetat) ist eine chemisch reduzierte Form des Fluorescein, die als Indikator für die reaktive Sauerstoffspezies (ROS) in Zellen verwendet wird, um z.B. die Generierung von reaktiven Sauerstoff-Zwischenprodukten in neutrophilen Granulozyten und Makrophagen zu erkennen. Nach der Abspaltung der Acetat-Gruppen durch intrazelluläre Esterasen und der Oxidation wird das nicht-fluoreszierende H₂DCFDA in das stark fluoreszierende 2',7'-Dichlorfluorescein (DCF) umgewandelt. Es stehen Versionen dieses Indikators zur Verfügung, die eine bessere zelluläre Retention aufweisen (C400, C2938).

Spezifikationen des ROS-Indikators:

• Ex/Em: ∼492 – 495/517 – 527 nm
• Produkt ist luftempfindlich und sollte unter trockenem Argon oder Stickstoff gelagert werden
• Produkt kann zur Verwendung in DMSO, DMF oder Ethanol gelöst werden
• Membrangängiger Indikator (Zellladeprotokolle sind in den Literaturhinweisen erhältlich)
• Die Fluoreszenz kann durch Durchflusszytometer, Fluorometer, Mikrotiterplatten-Lesegeräte oder Fluoreszenzmikroskope mit Anregungsquellen und Filtern für Fluorescein überwacht werden

Weitere ROS-Indikatoren
Wir bieten ein Sortiment an Molecular Probes™ Produkten für die Herstellung reaktiver Sauerstoffspezies (ROS) an, einschließlich Singulett-Sauerstoff, Superoxid, Hydroxyl-Radikal und verschiedenen Peroxiden und Hydroperoxiden sowie für deren fluorometrischen Nachweis in Lösung. Weitere Informationen zu diesen Produkten finden Sie im Abschnitt Herstellung und Nachweis reaktiver Sauerstoffspezies – Abschnitt 18.2 im Molecular Probes™ Handbuch.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Menge100 mg

ProdukttypROS Indicator

Unit SizeEach

Häufig gestellte Fragen (FAQ)

I want to assay cells for reactive oxygen species using carboxy-H2DCFDA, but I want to do so with a plate reader instead of microscope. Will it work?

It has been done. The problem is that plate readers are less sensitive than microscopes, with far less signal-to-background difference. It is worth trying, but first optimize concentrations and loading times with control cells, use a plate with little to no autofluorescence, and possibly optimize the gain setting in order to get the best signal possible. But don't expect the same sensitivity, even with optimization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I have GFP-transfected cells and need to label for reactive oxygen species. Can I use H2DCFDA?

This is not recommended as the two dyes overlap in the emission wavelength. There are other ROS reagents available in different wavelengths, such as CellROX Deep Red, which emits in the far-red range (665 nm), or dihydroethidium, which is emits in the visible red range (620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my cell with CM-H2DCFDA for reactive oxygen detection, but upon illuminating the cell there is a significant increase in fluorescence in the control cells. Why?

If the cell is overloaded with dye, the high intracellular concentration of the dye may lead to dye-dye quenching. Upon illumination, photobleaching will occur, which will reduce the dye-dye quenching and actually increase the fluorescence (for a while, but then it will start decreasing). To solve the problem, reduce the concentration and incubation time, and try a range of incubation times and concentrations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2 DCFDA fixable?

H₂DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX Deep Red and CellROX Green are retained for a limited time upon fixation with formaldehyde. CellROX Green may be retained upon subsequent Triton X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the recommended storage condition/shelf-life for H2DCFDA (H2-DCF, DCF) (Cat. No. D399)?

We guarantee stability of the product for a period of 3 months when stored as recommended in the freezer (-5 to -30 degrees C).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all H2DCFDA (H2-DCF, DCF) FAQs

Zitierungen und Referenzen (1016)

Zitierungen und Referenzen

Abstract

Nitric oxide protects cultured rat hepatocytes from tumor necrosis factor-alpha-induced apoptosis by inducing heat shock protein 70 expression.

Authors:Kim YM,de Vera ME,Watkins SC,Billiar TR

Journal:The Journal of biological chemistry

PubMed ID:8995451

Differing effects of copper,zinc superoxide dismutase overexpression on neurotoxicity elicited by nitric oxide, reactive oxygen species, and excitotoxins.

Authors:Ying W,Anderson CM,Chen Y,Stein BA,Fahlman CS,Copin JC,Chan PH,Swanson RA

Journal:Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

PubMed ID:10698074

Authors:

Journal:

PubMed ID:11282963

Imaging of hydroperoxides in a rat glomerulus stimulated by puromycin aminonucleoside.

Authors:Aoyagi K,Akiyama K,Tomida C,Gotoh M,Hirayama A,Takemura K,Ueda A,Nagase S,Koyama A,Narita M

Journal:Kidney international. Supplement

PubMed ID:10412763

Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimodipine.

Authors:Danks AM,Hammond DN,Wainer BH,Van Buskirk RG,Isaacson RL

Journal:Brain research. Molecular brain research

PubMed ID:1334195

The immortalized septal cell line, SN56 B5 G4, generated by the fusion of mouse septal area cells and neuroblastoma cells, was used to determine if nimodipine, an antagonist of voltage sensitive calcium 'L' channels, might act in a neuroprotective fashion when intracellular calcium levels were raised by incubation in ouabain ... More

View all H2DCFDA (H2-DCF, DCF) citations