AlamarBlue® Cell Viability Reagent

Catalog number: DAL1100

Molecular Probes™  Related applications: Cell Counting, Viability, & Cryopreservation | Cell Viability, Proliferation & Function

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Description

The alamarBlue® dye is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Applications:

Cell proliferation determinations—the alamarBlue® dye is water soluble, stable in culture, and non-toxic
Cell viability assays, including cytotoxicity—metabolic activity and dye generation changes in proportion to altered viability and cell damage
Cytokine assays—measure cytokine-induced proliferation, recover and expand cells at the end of the study if desired

The alamarBlue® dye as a cell viability assay reagent
The alamarBlue® assay is based on the ability of metabolically active cells to convert the reagent into a fluorescent and colorimetric indicator. Damaged and non-viable cells have lower innate metabolic activity, and generate a proportionally lower signal. The dye itself has low toxicity, and is generally safe for cells.

A safe, convenient, and versatile assay
AlamarBlue® dye is an indigo-colored liquid that has been shown to effectively measure innate metabolic activity in animal, fungal and bacterial cells. Since alamarBlue® dye is non-toxic, the effector cells may be recovered for further analysis or cell expansion at the end of a study. AlamarBlue® dye is water-soluble, saving time otherwise spend on washing and extraction steps. The homogeneous assay format of this kit is fast—add the single reagent directly to cells in suspension or to attached cells in full medium. The protocol is readily amenable to automation and high-throughput assays. The ability to detect the alamarBlue® indicator by either fluorescence or spectroscopy provides compatibility with flow cytometry, plate readers and microscopy.

Experimental detection of alamarBlue® dye

Fluorometry: By excitation at 530–560 nm and emission at 590 nm
Spectrophotometry: By absorbance measurements at 570 nm and 600 nm
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Blue
For Use With (Equipment): Microplate Reader, Spectrophotometer
Format: 96-well plate, 384-well plate, Cuvettes
Label or Dye: Resazurin
Product Size: 100 mL
Incubation Time: 1 to 4 hrs (up to 24 hrs for low cell numbers)
Compatible Cells: Bacteria, Fungal Cells, Eukaryotic Cells
Detection Method: Fluorescent, Colorimetric
High Throughput Compatibility: High Throughput-Compatible
Excitation⁄Emission (nm): 530-560⁄590
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols

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