EnzChek® Phosphatase Assay Kit

Catalog number: E12020

Molecular Probes™  Related applications: Cellular Imaging | Pharma & Biopharma | Protein Assays and Analysis | Target & Lead Identification & Validation

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Description

Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes' patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567). DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ~1000 assays, using a reaction volume of 100 µL per assay. Fluorescence (excitation/emission maxima ~358/455 nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Phosphatase Assay
Color: Blue
Detection Method: Fluorescent
Excitation⁄Emission (nm): 360⁄460
For Use With (Equipment): Microplate Reader
Label or Dye: DiFMU
Product Size: 1,000 assays
Substrate: DiFMUP
Substrate Properties: Chemical Substrate
Substrate Type: Phosphatase Substrate
Target Enzyme: Phosphatases
Technique: Fluorescence Intensity
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols