EnzChek™ Phosphatase Assay Kit

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}} also known as {{product.formattedSku}} 
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes' patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567). DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ~1000 assays, using a reaction volume of 100 µL per assay. Fluorescence (excitation/emission maxima ~358/455 nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
For Research Use Only. Not for use in diagnostic procedures.


Assay Type: Phosphatase Assay
Color: Blue
Detection Method: Fluorescent
Excitation⁄Emission (nm): 360⁄460
For Use With (Equipment): Microplate Reader
Label or Dye: DiFMU
Product Line: EnzChek®
Product Size: 1,000 assays
Substrate: DiFMUP
Substrate Properties: Chemical Substrate
Substrate Type: Phosphatase Substrate
Target Enzyme: Phosphatases
Technique: Fluorescence Intensity
Shipping Condition: Room Temperature

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols