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EnzChek® Myeloperoxidase (MPO) Activity Assay Kit

Catalog number: E33856

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Protein Assays and Analysis

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Description

Myeloperoxidase (MPO) is a unique peroxidase that, in addition to its peroxidation activity, also catalyzes the conversion of hydrogen peroxide (H2O2) and chloride (Cl-) to hypochlorous acid (HOCl). The EnzChek® Myeloperoxidase (MPO) Activity Assay Kit provides assays for the determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates. For detection of chlorination, the kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (-OCl) to yield fluorescein. Peroxidation is detected using nonfluorescent Amplex® UltraRed reagent (A36006), which is oxidized by the H2O2-generated redox intermediates MPO-I and MPO-II to form a fluorescent product. The EnzChek® Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over a broad dynamic range (1.5 to 200 ng/mL). The speed (30 minutes), sensitivity, and mix-and-read convenience make this kit ideal for measuring MPO activities and for high-throughput screening for MPO-specific inhibitors.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Fluorescence Intensity
Assay Type: Myeloperoxidase (MPO) Assay
Product Size: 1 kit
Target Enzyme: Myeloperoxidase
Substrate Properties: Chemical Substrate
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

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