Gibco™

Novex™ pH 3-7 IEF Protein Gels, 1.0 mm, 10-well

Catalog number: EC6645BOX
Gibco™

Novex™ pH 3-7 IEF Protein Gels, 1.0 mm, 10-well

Catalog number: EC6645BOX
Catalog Number
EC6645BOX
Unit Size
5 gels (1 box)
Price (USD)
225.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
EC6645BOX5 gels (1 box)
225.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Novex® pH 3–7 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.

IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.

Formulation
Novex® IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.

Recommended buffers
IEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Size
Mini
Separation Range
pH 3.5 to 6.8
Gel Percentage
5%
Gel Type
IEF
Gel Thickness
1.0 mm
For Use With (Equipment)
Mini Gel Tank, XCell SureLock Mini
Separation of
Protein
Wells
10-well
Quantity
5 gels
Mode of Separation
pI (Charge)
Shipping Condition
Wet Ice
System Type
Novex™
Well Design
1D Well
Product Line
Novex™
Product Type Specs
IEF Protein Gel
Shelf Life
6 Months
pH Range
3 to 7
Sample Loading Volume
25 µL

Contents & Storage

One box contains 5 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life up to 6 months.

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Frequently asked questions (FAQs)

Citations & References