Invitrogen™

Novex™ 10% Tricine Protein Gels, 1.0 mm, 10-well

Catalog number:  EC6675BOX

Related applications:

Protein Electrophoresis & Western Blotting

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Description

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Features of Novex Tricine protein gels:
• Increased resolution of proteins with molecular weights as low as 2 kDa
• Improved compatibility with direct sequencing of proteins after transferring to PVDF
• Minimized protein modification due to the lower pH of the tricine buffering system

Learn more about our Tricine gels ›

View migration charts ›

Formulation: Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation
Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer and optimal separation use Tricine SDS Running Buffer.

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer if performing a traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Specialized Protein Gel Support
Protein Gel 1D Electrophoresis Support

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Mini Gel Tank,XCell SureLock™ Mini
Gel Percentage: 10 %
Gel Size: Mini (8 cm x 8 cm)
Gel Type: Tricine Gel
Mode of Separation: Molecular Weight
Product Line: Novex™
Product Size: 10 gels (1 box)
Quantity: 10 gels
Recommended Applications: Denaturing
Sample Loading Volume: up to 25 µL
Separation Range: 10 to 30 kDa
Shipping Condition: Wet Ice
System: Novex®
Thickness: 1.0 mm
Well Format: 1D Well
Wells: 10 wells

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 4–8 weeks depending upon gel type.