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Invitrogen
Human Ferritin Rapid ELISA Kit
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Product Specifications
Analytical sensitivity
24.31 pg/mL
Assay range
62.5-4,000 pg/mL
Sample type/volume
Plasma
50 µL
Serum
50 µL
Hands-on time
15 min
Time-to-result
1 hr 30 min
Homogenous (no wash)
No
Interassay CV
<10%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate, Standard, HRP Conjugate, Standard & Sample Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
Shipping conditions
Wet ice
Storage
Refer to product documentation for component specific storage temperature.
Protein name
Ferritin
Species (tested)
Human
Species (reported)
Human
Assay kit format
Rapid ELISA Kit
Detector antibody conjugate
HRP
Label or dye
HRP
About This Kit
The Human Ferritin (FE) ELISA quantitates FE in serum and plasma.
Principle of the method
The Human FE solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme antibody target complex to produce measurable signal. The enzyme substrate reaction is terminated by the addition of stop solution and the color turns yellow. The intensity of this signal, measured spectrophotometrically at a wavelength of 450±2 nm, is directly proportional to the concentration of target present in the original specimen.
Achieve fast and reliable results in just 90 minutes with our Rapid ELISA kit, featuring a streamlined protocol that requires only one wash step.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Target information
Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : FHC, FTH, FTH1, FTHL6, FTL, FTL1, HFE5, LFTD, NBIA3, NBIA9, OK/SW-cl.84, PIG15, PLIF
Gene ID : (Human) 2495, (Human) 2512
Gene symbol : FTH1, FTL
Protein Aliases : apoferritin, Cell proliferation-inducing gene 15 protein, epididymis secretory sperm binding protein, Ferritin H subunit, Ferritin heavy chain, ferritin heavy subunit, Ferritin L subunit, ferritin L-chain, Ferritin light chain, ferritin light polypeptide-like 3, ferritin light subunit, ferritin, heavy polypeptide 1, light polypeptide, frih, fril, L apoferritin, MGC71996, FTL, H-ferritin, iron ion binding, iron-binding, immunosuppressor, growth factor, PIG15, placenta immunoregulatory factor, proliferation-inducing protein 15, unnamed protein product
UniProt ID (Human) P02794, (Human) P02792
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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