IL-2 ELISA Kit, Human

Catalog number: EH2IL2

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Use the Thermo Scientific® Human IL-2 ELISA Kit to assay IL-2 (human interleukin-2) levels in human serum, plasma (with EDTA, heparin, sodium citrate), urine, and cell culture supernatant samples. This is an alternate product for Cat. Nos. KHC0021 and KAC1241.

This kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of IL-2 levels in samples. The Thermo Scientific® Human IL-2 ELISA Kit also includes calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution, and stop solution. This complete kit contains one pre-coated 96-well strip plate and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

Highlights:
• Target: natural and recombinant human interleukin-2 (IL-2)
• Assay range: 38.4 to 1500 pg/mL
• Sensitivity: <6 pg/mL
• Sample types: cell culture supernatant, serum, plasma (with EDTA, sodium citrate, heparin)
• Total assay time: 4 hours
• Sample size: 50 μL
• Specificity: no significant cross-reaction with human IL-1α, IL-1β, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ, GM-CSF, INFγ, or mouse IL-2
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gene Aliases: IL-2, TCGF, lymphokine
Accession Number: NP_000577.2, NM_000586.3
Gene ID: 3558
Species: Human
Product Size: 96 assays
Protein Family: Cytokines & Receptors
Detection Method: Colorimetric
Research Category: Immunology

Contents & storage

Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.

Documents

Manuals & protocols