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Invitrogen
Human Mcl-1/Bcl2L3 ELISA Kit
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Product Specifications
Analytical sensitivity
0.13 ng/mL
Assay range
0.13-25 ng/mL
Sample type/volume
Supernatant
50 µL
Serum
50 µL
Plasma
50 µL
Hands-on time
1 hr 20 min
Time-to-result
4 hr 45 min
Homogenous (no wash)
No
Interassay CV
<12%
Intraassay CV
<10%
Instrument
Absorbance Microplate Reader
Product size
96 Tests
Contents
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Shipping conditions
Wet ice
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Protein name
MCL-1
Species (tested)
Human
Detector antibody conjugate
Biotin
Label or dye
HRP
About This Kit
Human Mcl-1/Bcl2L3 quantitates human Mcl-1/Bcl2L3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Mcl-1/Bcl2L3.
Target information
MCL1 (Myeloid cell leukemia-1) belongs to the Bcl-2 family and is involved in programing, differentiation and concomitant maintenance of cell viability, but not of proliferation. Isoform 1 of MCL1 inhibits apoptosis while isoform 2 promotes it. The carboxy terminal of MCL1 and bcl-2 share significant sequence homology. Expression of MCL1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of MCL1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, MCL1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis. While the mechanism by which MCL1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak. MCL1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. MCL1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals. MCL1 exists as at least two distinct isoforms designated MCL1L and MCL1S. In marked contrast to the larger isoform of MCL1, overexpression of MCL1S promotes cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Gene aliases : bcl2-L-3, BCL2L3, EAT, Mcl-1, MCL1-ES, mcl1/EAT, MCL1L, MCL1S, TM
Gene ID : (Human) 4170
Gene symbol : MCL1
Protein Aliases : anti-apoptotic, bcl-2-like protein 3, bcl-2-related protein EAT/mcl1, BCL2 family apoptosis regulator, MCL-1ES, MCL-1S, MGC104264, MGC1839, Mcl-1S, Bcl-2 family protein, proapoptotic protein, MCL1, myeloid cell leukemia 1, myeloid cell leukemia ES, myeloid cell leukemia sequence 1 (BCL2-related), unnamed protein product
UniProt ID (Human) Q07820
Performance Guarantee
If an Invitrogen™ immunoassay doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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